Team:LMU-Munich/Bacillus BioBricks/vector use

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* <p align="justify">''sacA''-locus and ''lacA''-locus: for those two loci, a colony PCR should be performed. The protocol can be found on our website. As shown below with an examplary locus, one of the primers of each pair is located on the genome, facing inwards, and the other one is located in between the recombination sites, facing outwards.</p>
* <p align="justify">''sacA''-locus and ''lacA''-locus: for those two loci, a colony PCR should be performed. The protocol can be found on our website. As shown below with an examplary locus, one of the primers of each pair is located on the genome, facing inwards, and the other one is located in between the recombination sites, facing outwards.</p>
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* <p align="justify">For sacA: you can use the following primers: up: CTGATTGGCATGGCGATTGC together with ACAGCTCCAGATCCTCTACG as well as down: GTCGCTACCATTACCAGTTG together with TCCAAACATTCCGGTGTTATC.
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* <p align="justify">For sacA: you can use the following primers: up: TM2505: CTGATTGGCATGGCGATTGC together with TM2506: ACAGCTCCAGATCCTCTACG as well as down: TM2507: GTCGCTACCATTACCAGTTG together with TM2508: TCCAAACATTCCGGTGTTATC.
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</p>
[[File:LMU-Munich-Colony_pcr.png|600px|center]]
[[File:LMU-Munich-Colony_pcr.png|600px|center]]
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* <p align="justify"> for ''lacA'', you can use the primers: TM2624: and TM2625: (result: 650 bp)
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* <p align="justify"> for ''lacA'', you can use the primers: TM2624: GAACGAAGGGCTAAGAGAAC
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as well as TM2624 and TM2627: (result: 3000 bp+ length of construct)
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and TM2625:AAGCAGAAGGCCATCCTGAC (result: 650 bp)as well as TM2624 and TM2627: AAGAATCCGCCCATATCGAG (result: 3000 bp+ length of construct)
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With colony PCR you can check any other locus.
With colony PCR you can check any other locus.

Latest revision as of 18:07, 1 August 2013

iGEM Ludwig-Maximilians-Universität München Beadzillus

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