Team:LMU-Munich/Application

From 2012.igem.org

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<p align="justify">Our [http://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins '''Sporo'''beads] offer a wide variety of applications within the laboratory, and around the world. '''Sporo'''beads can be used for filtration and protein screening.</p>
<p align="justify">Our [http://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins '''Sporo'''beads] offer a wide variety of applications within the laboratory, and around the world. '''Sporo'''beads can be used for filtration and protein screening.</p>
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====Filtration====
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|<p align="justify">Further information for the first possible application for our '''Sporo'''beads.</p>
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|[[File:Tabelle.png|right|150px|link=Team:LMU-Munich/Application/Filtration]]
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==Filtration==
==Filtration==
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<p align="justify">One example of such a filtering protein could be a CPX-'''Sporo'''bead. [http://partsregistry.org/wiki/index.php/Part:BBa_I728500 CPX] is a peptide developed by the [http://2007.igem.org/MIT 2007 MIT iGEM team], which is extremely hydrophilic, and thus capable of binding microparticles of polystyrene from water. The excessive use of disposable plastic and the lack of universal recycling programs has led to the [http://www.ncbi.nlm.nih.gov/pubmed/22610295 pollution of the world's oceans]. In the ocean, large pieces of plastic litter are ground by sea currents and degraded by UV radiation into microscopic pieces, so called "plastic plankton," which is consumed by fish, filter feeders, and other marine organisms. Such plastic uptake can lead to poisoning, sterility and death.</p>
<p align="justify">One example of such a filtering protein could be a CPX-'''Sporo'''bead. [http://partsregistry.org/wiki/index.php/Part:BBa_I728500 CPX] is a peptide developed by the [http://2007.igem.org/MIT 2007 MIT iGEM team], which is extremely hydrophilic, and thus capable of binding microparticles of polystyrene from water. The excessive use of disposable plastic and the lack of universal recycling programs has led to the [http://www.ncbi.nlm.nih.gov/pubmed/22610295 pollution of the world's oceans]. In the ocean, large pieces of plastic litter are ground by sea currents and degraded by UV radiation into microscopic pieces, so called "plastic plankton," which is consumed by fish, filter feeders, and other marine organisms. Such plastic uptake can lead to poisoning, sterility and death.</p>
<p align="justify">CPX-'''Sporo'''beads in huge filter boxes could be put into place to mechanically filter microscopic plastic particles out of the water. Such specific filtration would be superior to blanket filtration systems, which also remove living phytoplankton important to ocean ecosystems. To prevent the beads from being released into the sea and to ensure the plastic be removed from the water, the '''Sporo'''beads could be attached to membranes in the filter boxes. Then the '''Sporo'''beads would need to not only display CPX but also a membrane binding protein on their surface.</p>
<p align="justify">CPX-'''Sporo'''beads in huge filter boxes could be put into place to mechanically filter microscopic plastic particles out of the water. Such specific filtration would be superior to blanket filtration systems, which also remove living phytoplankton important to ocean ecosystems. To prevent the beads from being released into the sea and to ensure the plastic be removed from the water, the '''Sporo'''beads could be attached to membranes in the filter boxes. Then the '''Sporo'''beads would need to not only display CPX but also a membrane binding protein on their surface.</p>
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<div class="box">
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====Protein Screening====
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{| width="100%" style="text-align:center;"|
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|<p align="justify">Further information for the possible application of protein screening.</p>
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|[[File:Tabelle.png|right|150px|link=Team:LMU-Munich/Application/Protein Screening]]
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Application/Protein Screening]]
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==Protein Screening==
==Protein Screening==
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====Further Applications====
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|<p align="justify">Further information for the possible application of protein screening.</p>
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|[[File:Tabelle.png|right|150px|link=Team:LMU-Munich/Application/Further Applications]]
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Application/Further Applications]]
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==Further Applications==
==Further Applications==
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<p align="justify">There are many possible applications for our [http://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins '''Sporo'''beads], as it is possible to display all kinds of different proteins on their surface. To easily create them, we designed a [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks/Sporovector '''Sporo'''vector] in which you just have to insert the gene encoding your fusion protein of choice. Since there are so many possible applications, we illustrate three examplary ideas for future '''Sporo'''beads in the following section:</p>
<p align="justify">There are many possible applications for our [http://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins '''Sporo'''beads], as it is possible to display all kinds of different proteins on their surface. To easily create them, we designed a [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks/Sporovector '''Sporo'''vector] in which you just have to insert the gene encoding your fusion protein of choice. Since there are so many possible applications, we illustrate three examplary ideas for future '''Sporo'''beads in the following section:</p>
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Revision as of 18:44, 25 October 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU Photo2.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

[ more news ]

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