Team:LMU-Munich

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|<font size="5">'''Opening ''Bacillus subtilis'' and its unique biology to the iGEM world'''</font>
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:LMU-Munich_logo.png|200px|right|frame]]
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''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:LMU-Munich_team.png|right|frame|Your team picture]]
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|align="center"|[[Team:LMU-Munich | Team LMU-Munich]]
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<div class="fullbox">
 
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== BEADzillus ==
 
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We want to create Bacillus subtilis spores with different hybrid proteins on the outmost spore coat layer for multiple purposes.
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[[File:Prezi Home.png|600px|link=Team:LMU-Munich/Project]]
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As an safety issue, we want to disable the spores from germination.
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To provide the partsregistry with thoroughly evaluted biobricks we want to adapt B. subtilis vectors compatible for the BioBrick system and characterize different promoters.
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{|
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|[[File:NEXT.png|100px|link=Team:LMU-Munich/Abstract]]
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|'''Bead'''zillus - For a guided tour through the most important information about our project and results, klick [[Team:LMU-Munich/Abstract|here]].
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|[[File:LMU_Why_BS.png|100px|link=Team:LMU-Munich/Why_Beadzillus]]
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|Why is there a need for our Project [[Team:LMU-Munich/Why_Beadzillus|'''Bead'''zillus]]?
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|[[File:LMU_The_solution.png|100px|link=Team:LMU-Munich/Bacillus_Introduction]]
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|Why we choose to work with [[Team:LMU-Munich/Bacillus_Introduction|''Bacillus subtilis'']].
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|[[File:Requirements.png|95px|link=Team:LMU-Munich/Bacillus_BioBricks]]
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|To carry out our project we first to had to create a [[Team:LMU-Munich/Bacillus_BioBricks|<b><i>Bacillus</i>B</b>io<b>B</b>rick<b>B</b>ox]] and had to think over important [[Team:LMU-Munich/Germination_Stop|security issues]].  
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|[[File:4-Sporobeads.png|95px|link=Team:LMU-Munich/Spore_Coat_Proteins]]
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|Finally, we succeeded in displaying proteins on endospores. Our [[Team:LMU-Munich/Spore_Coat_Proteins|'''Sporo'''beads]].
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|[[File:LMU 5-Application.png|90px|link=Team:LMU-Munich/Application]]
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|With our '''Sporo'''beads a lot of [[Team:LMU-Munich/Application|applications]] are imaginable.
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<!--- The Mission, Experiments --->
 
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==='''Bead'''zillus: Fundamental BioBricks for ''Bacillus subtilis'' and spores as a platform for protein display===
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!align="center"|[[Team:LMU-Munich|Home]]
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!align="center"|[[Team:LMU-Munich/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=LMU-Munich Official Team Profile]
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We chose to work with [https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction <i>Bacillus subtilis</i>] to set new horizons and offer tools for this model organism to the <i>Escherichia coli</i>-dominated world of iGEM. Therefore, we created a [https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks <b><i>Bacillus</i>B</b>io<b>B</b>rick<b>B</b>ox] (<b>B<sup>4</sup></b>) composed of reporter genes, defined promoters, as well as reporter, expression, and empty vectors in BioBrick standard. <i>B. subtilis</i> naturally produces stress-resistant endospores which can germinate in response to suitable environmental conditions. To highlight this unique feature using the <b>B<sup>4</sup></b>, we developed [https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins <b>Sporo</b>beads]. These are spores displaying fusion proteins on their surface. As a proof of principle, we fused GFP to the outermost layer. Expanding this idea, we designed a [https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks/Sporovector <b>Sporo</b>vector] to easily create any <b>Sporo</b>bead imaginable. Because the <b>Sporo</b>beads must be biologically safe and stable vehicles, we [https://2012.igem.org/Team:LMU-Munich/Germination_Stop prevented germination] by knocking out involved genes and developed a [https://2012.igem.org/Team:LMU-Munich/Germination_Stop <b>Suicide</b>switch] turned on in case of germination. With the project <b>Bead</b>zillus, our team demonstrates the powerful nature of <i>B. subtilis</i>.
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!align="center"|[[Team:LMU-Munich/Project|Project]]
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!align="center"|[[Team:LMU-Munich/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:LMU-Munich/Modeling|Modeling]]
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!align="center"|[[Team:LMU-Munich/Notebook|Notebook]]
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!align="center"|[[Team:LMU-Munich/Safety|Safety]]
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====Team LMU Munich 2012====
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!align="center"|[[Team:LMU-Munich/Attributions|Attributions]]
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We are the iGEM team of the Ludwig-Maximilians-University (LMU) in Munich. The team consists of 10 dedicated members: 7 students and our 3 advisors. Visit our [https://2012.igem.org/Team:LMU-Munich/Team Team] page for more Details.
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|<html>
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<a href="http://www.en.uni-muenchen.de/index.html">
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<img src="https://static.igem.org/mediawiki/2012/8/85/LMU-Munich_logo.png" height="170" border=0 /></a></html>
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<a href="http://www.biologie.uni-muenchen.de/index.html">
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<img src="https://static.igem.org/mediawiki/2012/4/48/Biozentrum_LMU.jpg" height="170" border=0 /></a></html>
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<a href="https://2012.igem.org/Team:LMU-Munich/Team">
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<img src="https://static.igem.org/mediawiki/2012/1/13/LMU-Munich_team2.png" height="350" border=0 align="right"/></a></html>
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[[File:Uumail.png|80px|left]]
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Email us!
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igem@bio.lmu.de
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[[File:Facebook logo amsterdam.png|70px|left|link=https://www.facebook.com/igem.lmu]] Visit us on facebook
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[[File:Exe12_Youtubelogo.png|65px|left|link=http://www.youtube.com/user/igemLMUmunich]] Check out our videos!
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{| align="right"
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| [[Team:LMU-Munich/Team|Find out who we are!]]     
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====Our Gold Sponsors====
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<a href="https://2012.igem.org/Team:LMU-Munich/Sponsors">
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<img src="https://static.igem.org/mediawiki/2012/4/4b/LMU_GoldSponsors.png" width="650" border=0 />
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Latest revision as of 13:46, 26 October 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU Photo1.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

[ more news ]

Sporenfreunde

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