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Team:LMU-Munich
From 2012.igem.org
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|Beads are widely used in Biotechnology but... | |Beads are widely used in Biotechnology but... | ||
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|Endospores of Bacillus subtilis offer a great platform for biological beads | |Endospores of Bacillus subtilis offer a great platform for biological beads | ||
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|In order to achieve this we first had to create essential BioBricks for the work with ''B. subtilis'' and design a module to prevent germination of the spores. | |In order to achieve this we first had to create essential BioBricks for the work with ''B. subtilis'' and design a module to prevent germination of the spores. | ||
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Revision as of 22:47, 24 October 2012
The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".
[ more news ]
| Opening Bacillus subtilis and its unique biology to the iGEM world |
| Beads are widely used in Biotechnology but... |
| Endospores of Bacillus subtilis offer a great platform for biological beads |
| In order to achieve this we first had to create essential BioBricks for the work with B. subtilis and design a module to prevent germination of the spores. |
Beadzillus: Fundamental BioBricks for Bacillus subtilis and spores as a platform for protein display
We chose to work with Bacillus subtilis to set new horizons and offer tools for this model organism to the Escherichia coli-dominated world of iGEM. Therefore, we created a BacillusBioBrickBox (B4) composed of reporter genes, defined promoters, as well as reporter, expression, and empty vectors in BioBrick standard. B. subtilis naturally produces stress resistant endospores which can germinate in response to suitable environmental conditions. To highlight this unique feature using the B4, we developed Sporobeads. These are spores displaying fusion proteins on their surface. As a proof of principle, we fused GFP to the outermost layer. Expanding this idea, we designed a Sporovector to easily create any Sporobead imaginable. Because the Sporobeads must be biologically safe and stable vehicles, we prevented germination by knocking out involved genes and developed a Suicideswitch turned on in case of germination. With the project Beadzillus, our team demonstrates the powerful nature of B. subtilis.
Team LMU Munich 2012
We are the iGEM team of the Ludwig-Maximilians-University (LMU) in Munich. The team consists of 10 dedicated members: 7 students and our 3 advisors. Visit our Team page for more Details.
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igem@bio.lmu.de
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