Team:KIT-Kyoto/kazukokekokko

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Revision as of 11:07, 10 September 2012 by Kazuko (Talk | contribs)
LB medium
Bacto-tryptone10g
Bacto-yeast extract5g
NaCl10g
dH2O1000mL


LB plate
Bacto-tryptone10g
Bacto-yeast extract5g
NaCl10g
dH2O1000mL


Solution Ⅰ
Glucose50mM
EDTA10mM
Tris-HCl25mM


Solution Ⅱ
NaOH2N
SDS1%


Solution Ⅲ
K-acetate5M


BP reaction by invitrogen gateway system
attB DNA sample75ng/reaction
pDONR vector150ng/reaction
TE Bufferup
Total8uL or 9uL


LR reaction by invitrogen gateway system
BP DNA sample50-150ng/reaction
Destination vector150ng/reaction
TE Bufferup
Total8uL or 9uL

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