Team:KIT-Kyoto/kazukokekokko

From 2012.igem.org

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<td width="800px" height="510px" valign="top">
<td width="800px" height="510px" valign="top">
<div id="MIGI">
<div id="MIGI">
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LB medium
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<br>
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<Table Border Cellspacing="0">
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<Tr><Td>Bacto-tryptone</Td><Td>10g</Td></Tr>
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<Tr><Td>Bacto-yeast extract</Td><Td>5g</Td></Tr>
+
-
<Tr><Td>NaCl</Td><Td>10g</Td></Tr>
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-
<Tr><Td>dH<sub>2</sub>O</Td><Td>1000mL</Td></Tr>
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</Table>
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<br>
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<br>
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LB plate
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<br>
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<Table Border Cellspacing="0">
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<Tr><Td>Bacto-tryptone</Td><Td>10g</Td></Tr>
+
-
<Tr><Td>Bacto-yeast extract</Td><Td>5g</Td></Tr>
+
-
<Tr><Td>NaCl</Td><Td>10g</Td></Tr>
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-
<Tr><Td>dH<sub>2</sub>O</Td><Td>1000mL</Td></Tr>
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-
</Table>
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-
<br>
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<br>
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Solution Ⅰ
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<br>
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<Table Border Cellspacing="0">
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<Tr><Td>Glucose</Td><Td>50mM</Td></Tr>
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<Tr><Td>EDTA</Td><Td>10mM</Td></Tr>
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<Tr><Td>Tris-HCl</Td><Td>25mM</Td></Tr>
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</Table>
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<br>
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<br>
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Solution Ⅱ
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<br>
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<Table Border Cellspacing="0">
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-
<Tr><Td>NaOH</Td><Td>2N</Td></Tr>
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<Tr><Td>SDS</Td><Td>1%</Td></Tr>
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</Table>
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<br>
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<br>
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Solution Ⅲ
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<br>
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<Table Border Cellspacing="0">
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<Tr><Td>K-acetate</Td><Td>5M</Td></Tr>
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</Table>
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<br>
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<br>
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BP reaction by invitrogen gateway system
BP reaction by invitrogen gateway system
<Table Border Cellspacing="0">
<Table Border Cellspacing="0">

Revision as of 14:44, 14 September 2012







BP reaction by invitrogen gateway system
attB DNA sample75ng/reaction
pDONR vector150ng/reaction
TE Bufferup
Total8uL or 9uL


LR reaction by invitrogen gateway system
BP DNA sample50-150ng/reaction
Destination vector150ng/reaction
TE Bufferup
Total8uL or 9uL



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