Team:KIT-Kyoto/kazukokekokko

(Difference between revisions)

Revision as of 11:07, 10 September 2012

LB medium

LB plate

Solution Ⅰ

Solution Ⅱ

NaOH	2N
SDS	1%

Solution Ⅲ

K-acetate

5M

BP reaction by invitrogen gateway system

LR reaction by invitrogen gateway system

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@@ Line 78: / Line 78: @@
 <td width="800px" height="510px" valign="top">
 <div id="MIGI">
+LB medium
+<br>
+<Table Border Cellspacing="0">
+<Tr><Td>Bacto-tryptone</Td><Td>10g</Td></Tr>
+<Tr><Td>Bacto-yeast extract</Td><Td>5g</Td></Tr>
+<Tr><Td>NaCl</Td><Td>10g</Td></Tr>
+<Tr><Td>dH<sub>2</sub>O</Td><Td>1000mL</Td></Tr>
+</Table>
+<br>
+<br>
+LB plate
+<br>
+<Table Border Cellspacing="0">
+<Tr><Td>Bacto-tryptone</Td><Td>10g</Td></Tr>
+<Tr><Td>Bacto-yeast extract</Td><Td>5g</Td></Tr>
+<Tr><Td>NaCl</Td><Td>10g</Td></Tr>
+<Tr><Td>dH<sub>2</sub>O</Td><Td>1000mL</Td></Tr>
+</Table>
+<br>
+<br>
+Solution Ⅰ
+<br>
+<Table Border Cellspacing="0">
+<Tr><Td>Glucose</Td><Td>50mM</Td></Tr>
+<Tr><Td>EDTA</Td><Td>10mM</Td></Tr>
+<Tr><Td>Tris-HCl</Td><Td>25mM</Td></Tr>
+</Table>
+<br>
+<br>
+Solution Ⅱ
+<br>
+<Table Border Cellspacing="0">
+<Tr><Td>NaOH</Td><Td>2N</Td></Tr>
+<Tr><Td>SDS</Td><Td>1%</Td></Tr>
+</Table>
+<br>
+<br>
+Solution Ⅲ
+<br>
+<Table Border Cellspacing="0">
+<Tr><Td>K-acetate</Td><Td>5M</Td></Tr>
+</Table>
+<br>
+<br>
 BP reaction by invitrogen gateway system
 <Table Border Cellspacing="0">
@@ Line 90: / Line 134: @@
 <Table Border Cellspacing="0">
 <Tr><Td>BP DNA sample</Td><Td>50-150ng/reaction</Td></Tr>
-<Tr><Td＞Destination vector</Td><Td>150ng/reaction</Td></Tr>
+<tr><td>Destination vector</td><td>150ng/reaction</td></tr>
 <Tr><Td>TE Buffer</Td><Td>up</Td></Tr>
 <Tr><Td>Total</Td><Td>8uL or 9uL</Td></Tr>