Team:KIT-Kyoto/Notebook-week7

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<h2>September 17th</h2>
 
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<h2>September 18th</h2>
<h2>September 18th</h2>
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By red eye screening, we identified the strain 7 and strain 10 to be the successfully transformed lines. Photographs for the transformed red (yellow) eye fly (strain 10) were taken.
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<img src="http://2012.igem.org/wiki/images/c/c1/HAEE2.JPG" width="320" height="220">
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<h2>September 19th</h2>
<h2>September 19th</h2>
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By red eye screening, we identified the strain 13 and strain 14 to be the successfully transformed lines. Photographs for the transformed red (orange) eye fly (strain 13) were taken.
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<img src="http://2012.igem.org/wiki/images/a/a6/Strain13.JPG" width="320" height="220">
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Drosophila S2 cells (2.5 X 10<sup>5</sup> cells per well) were plated on the 24 well plate and cultured in the Schneider’s Drosophila medium containing 10% fetal bovine serum at 25 ℃.
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<h2>September 20th</h2>
<h2>September 20th</h2>
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By red eye screening, we identified the strain 23 and strain 29 to be the successfully transformed lines.
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<h2>September 21st</h2>
<h2>September 21st</h2>
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Red eye screening was continued.
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<h2>September 22nd</h2>
<h2>September 22nd</h2>
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Red eye screening was continued.
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<br><br>
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<h2>September 23rd</h2>
<h2>September 23rd</h2>
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By red eye screening, we identified the strain 56 and strain 65 to be the successfully transformed lines. These transformed flies are kept at 25℃ and later will be further inspected for chromosome linkage of the transgene.
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Latest revision as of 02:38, 27 September 2012






September 18th


By red eye screening, we identified the strain 7 and strain 10 to be the successfully transformed lines. Photographs for the transformed red (yellow) eye fly (strain 10) were taken.



September 19th


By red eye screening, we identified the strain 13 and strain 14 to be the successfully transformed lines. Photographs for the transformed red (orange) eye fly (strain 13) were taken.



Drosophila S2 cells (2.5 X 105 cells per well) were plated on the 24 well plate and cultured in the Schneider’s Drosophila medium containing 10% fetal bovine serum at 25 ℃.

September 20th


By red eye screening, we identified the strain 23 and strain 29 to be the successfully transformed lines.

September 21st


Red eye screening was continued.

September 22nd


Red eye screening was continued.

September 23rd


By red eye screening, we identified the strain 56 and strain 65 to be the successfully transformed lines. These transformed flies are kept at 25℃ and later will be further inspected for chromosome linkage of the transgene.