Team:KIT-Kyoto/Notebook-week6
From 2012.igem.org
(Difference between revisions)
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<h2>September 11th</h2> | <h2>September 11th</h2> | ||
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+ | <strong>TNFA and API2</strong> | ||
+ | <br><br> | ||
The female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃. | The female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃. | ||
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<strong>Parts</strong> | <strong>Parts</strong> | ||
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<h2>September 12th</h2> | <h2>September 12th</h2> | ||
<br> | <br> | ||
+ | <strong>TNFA and API2</strong> | ||
+ | <br><br> | ||
The female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃. | The female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃. | ||
<br><br> | <br><br> | ||
+ | |||
+ | |||
<strong>Parts</strong> | <strong>Parts</strong> | ||
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<h2>September 13th</h2> | <h2>September 13th</h2> | ||
<br> | <br> | ||
+ | <strong>TNFA and API2</strong> | ||
+ | <br><br> | ||
The female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃. | The female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃. | ||
<br><br> | <br><br> | ||
+ | |||
+ | |||
<strong>Parts</strong> | <strong>Parts</strong> | ||
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<h2>September 14th</h2> | <h2>September 14th</h2> | ||
<br> | <br> | ||
+ | <strong>TNFA and API2</strong> | ||
+ | <br><br> | ||
The adult male and virgin female flies from microinjected embryos were mated with yw virgin female flies and yw male flies, respectively. Mated flies were transferred to the new food vials in every three days to lay eggs as much as possible. The female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃. | The adult male and virgin female flies from microinjected embryos were mated with yw virgin female flies and yw male flies, respectively. Mated flies were transferred to the new food vials in every three days to lay eggs as much as possible. The female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃. | ||
<br><br> | <br><br> | ||
+ | |||
+ | |||
<strong>Parts</strong> | <strong>Parts</strong> | ||
<br><br> | <br><br> | ||
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<h2>September 15th</h2> | <h2>September 15th</h2> | ||
<br> | <br> | ||
+ | <strong>TNFA and API2</strong> | ||
+ | <br><br> | ||
The female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃. | The female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃. | ||
<br><br> | <br><br> | ||
+ | |||
+ | |||
<strong>Parts</strong> | <strong>Parts</strong> | ||
<br><br> | <br><br> | ||
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<h2>September 16th</h2> | <h2>September 16th</h2> | ||
<BR> | <BR> | ||
+ | <strong>TNFA and API2</strong> | ||
+ | <br><br> | ||
The adult male and virgin female flies from microinjected embryos were mated with yw virgin female flies and yw male flies, respectively. In total 83 flies from microinjected embryos were mated with yw flies. Mated flies were transferred to the new food vials in every three days to lay eggs as much as possible. | The adult male and virgin female flies from microinjected embryos were mated with yw virgin female flies and yw male flies, respectively. In total 83 flies from microinjected embryos were mated with yw flies. Mated flies were transferred to the new food vials in every three days to lay eggs as much as possible. | ||
<br><br> | <br><br> | ||
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<h2>September 17th</h2> | <h2>September 17th</h2> | ||
<BR> | <BR> | ||
+ | <strong>TNFA and API2</strong> | ||
+ | <br><br> | ||
The progeny flies were inspected by dissecting microscope to look for the successfully transformed w+ red eye flies (red eye screening). However, no red eye fly was found. | The progeny flies were inspected by dissecting microscope to look for the successfully transformed w+ red eye flies (red eye screening). However, no red eye fly was found. | ||
<br><br> | <br><br> | ||
+ | |||
+ | |||
<strong>Parts</strong> | <strong>Parts</strong> | ||
<br><br> | <br><br> |
Revision as of 14:53, 23 September 2012
September 11thTNFA and API2 The female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃. Parts
September 12thTNFA and API2 The female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃. Parts
September 13thTNFA and API2 The female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃. Parts
September 14thTNFA and API2 The adult male and virgin female flies from microinjected embryos were mated with yw virgin female flies and yw male flies, respectively. Mated flies were transferred to the new food vials in every three days to lay eggs as much as possible. The female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃. Parts
September 15thTNFA and API2 The female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃. Parts
September 16thTNFA and API2 The adult male and virgin female flies from microinjected embryos were mated with yw virgin female flies and yw male flies, respectively. In total 83 flies from microinjected embryos were mated with yw flies. Mated flies were transferred to the new food vials in every three days to lay eggs as much as possible. September 17thTNFA and API2 The progeny flies were inspected by dissecting microscope to look for the successfully transformed w+ red eye flies (red eye screening). However, no red eye fly was found. Parts
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