Team:KAIT Japan/Results

From 2012.igem.org

(Difference between revisions)
(methylation site of tar)
(methylation site of tar)
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:'''8.All production can be seen the band of cytoplasmic region of tar. So,These miniprep production are tar parts.'''
:'''8.All production can be seen the band of cytoplasmic region of tar. So,These miniprep production are tar parts.'''
:'''methylation site of tar is 690bp'''
:'''methylation site of tar is 690bp'''
-
:[[File:KAIT_Japan-Results-Met3.jpg]]
 
-
:'''Figure3 PCR production by using VF and butVR'''
 
:[[File:KAIT_Japan-Results-Met4.jpg]]
:[[File:KAIT_Japan-Results-Met4.jpg]]
 +
:'''Figure3 PCR production by using VF and butVR'''

Revision as of 16:57, 26 September 2012

KAIT Japan2012 logo.png
KAIT Japan2012 Sub.png
Kaitjapan iGEM official.logo.png


Kaitjapan.home.png

Home

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Project

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Parts

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Protocol

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Notebook

Kaitjapan results.png

Results

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Safety

Kaitjapan human practice.png

Human Practice

Kaitjapan team.png

Team

Results

cytoplasmic region of tar

We did PCR so as to get cytoplasmic region of tar.We used primer with restriction.
KAIT Japan-Results-Tar1.jpg
Figure1.PCR production from BBa_J22010
enzyme tag EcoRⅠand PstⅠ We experimented as following.
1.restriction enzyme processing PCR production
2.ligation
3.transformation
4.culture on the LB nutrient medium and get colonies.
5.colony PCR and numbering colonies.
6.We did miniprep 1,2,4,5.this is colony number
KAIT Japan-Results-Tar2.jpg
Figure2.the colony having cytoplasmic region of tar
7.After miniprep we did PCR by using VF2 and VR primer
8.All production can be seen the band of cytoplasmic region of tar. So,These miniprep production are tar parts.
cytoplasmic region of tar bp is varied. 900bp~1000bp
KAIT Japan-Results-Tar3.jpg
Figure3 PCR production by using VF and butVR

methylation site of tar

We did PCR so as to get methylation site of tar.We used primer with restriction
KAIT Japan-Results-Met1.jpg
Figure1.PCR production from BBa_J22010
enzyme tag EcoRⅠand PstⅠ We experimented as following.
1.restriction enzyme processing PCR production
2.ligation
3.transformation
4.culture on the LB nutrient medium and get colonies.
5.colony PCR and numbering colonies.
6.We did miniprep 1,2,4,5.this is colony number
KAIT Japan-Results-Met2.jpg
Figure2. the colony having methylation site of tar
7.After miniprep we did PCR by using VF2 and VR primer
8.All production can be seen the band of cytoplasmic region of tar. So,These miniprep production are tar parts.
methylation site of tar is 690bp
KAIT Japan-Results-Met4.jpg
Figure3 PCR production by using VF and butVR