Team:KAIT Japan/Notebook

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Home

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Project

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Parts

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Protocol

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Notebook

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Results

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Safety

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Human Practice

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Team

Notebook

Creating parts of Tar methylation region

Date:8/9

Colony PCR

→Reflection:We Took E.coli too many.You should take less E.coli.
→Reflection:The number of cycles was less.So,We increased The number of cycles in 8/11.(50 cycles)

Date:8/11

The purified DNA

→Reflection:Band was less.

PCR Product


Date:8/13

Confirmed of electrophoresis by PCR product and Ligation of the TA vector


Date:8/14

Transformation


Date:8/25

Miniprep

Creating parts of azurin

Date:8/16

Colony PCR Ligation


Date:8/18

Colony PCR


Date:8/20

DNA extraction and purification of P.aeruginosa