Team:KAIT Japan/Notebook

From 2012.igem.org

(Difference between revisions)
(Creating parts of EnvZ)
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:<big>'''Date:9/20'''</big> Restriction enzyme processing
:<big>'''Date:9/20'''</big> Restriction enzyme processing
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:<big>'''Date:9/21'''</big> Ligation
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:<big>'''Date:9/21'''</big> Ligation and PCR,Elecrtophoresis
:<big>'''Date:9/23'''</big> PCR
:<big>'''Date:9/23'''</big> PCR

Revision as of 17:55, 26 September 2012

KAIT Japan2012 logo.png
KAIT Japan2012 Sub.png
Kaitjapan iGEM official.logo.png


Kaitjapan.home.png

Home

Kaitjapan project.png

Project

Kaitjapan parts.png

Parts

Kaitjapan protocol.png

Protocol

Kaitjapan notebook.png

Notebook

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Results

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Safety

Kaitjapan human practice.png

Human Practice

Kaitjapan team.png

Team

Notebook

Our team pushed forward an experiment while making trial and error every day.

Creating parts of Methylation

Creating parts of Tar

Date:9/13 PCR and Colony PCR
Date:9/14 Electrophoretic confirmation
Date:9/15 Restriction enzyme processing
Date:9/16 Electrophoresis of the restriction enzyme processing product and Restriction enzyme processing
Date:9/17 Refinement of DNA and Ligation,Transformation
Date:9/18 Making of a master plate and Preculture,Recultivation
Date:9/19 Colony PCR and Electrophoresis
Date:9/20 Ligation and Transformation
Date:9/21 Inoculation
Date:9/22 Making of a master plate and Colony PCR,Electrophoresis,Preculture
Date:9/23 Electrophoretic confirmation and Electrophoresis of the PCR
Date:9/24 Miniprep and Electrophoresis
Date:9/25 PCR of the miniprep product and Electrophoresis

Creating parts of EnvZ

Date:9/10 Design of the primer and PCR,Elecrtophoresis,Miniprep
Date:9/13 PCR and Elecrtophoresis
Date:9/18 Design of the primer
Date:9/19 PCR and Elecrtophoresis
Date:9/20 Restriction enzyme processing
Date:9/21 Ligation and PCR,Elecrtophoresis
Date:9/23 PCR
Date:9/24 The refinement of DNA and Restriction enzyme processing,Ligation
Date:9/25 Transformation
Date:9/26 Liquid culture

Creating parts of Azurin

Creating parts of Tar methylation region

Date:8/9 Colony PCR
Date:8/11 The purified DNA
Date:8/13 Confirmed of electrophoresis by PCR product and Ligation of the TA vector
Date:8/14 Transformation
Date:8/25 Miniprep
Date:8/30 Electrophoresis,Refinement of DNA,PCR,Nanodrop
Date:9/1 Miniprep,PCR,Elecrtophoresis
Date:9/2 Meeting
Date:9/5 Miniprep,Nanodrop
Date:9/6 Miniprep,PCR,Electrophoresis
Date:9/10 Colony PCR,Refinement of DNA,c,Miniprep
Date:9/11 Primer design
Date:9/12 Miniprep,Ligation
Date:9/13 Electrophoresis,Refinement of DNA
Date:9/14 Colony PCR
Date:9/15 Ligation
Date:9/16 PCR,Ligation
Date:9/17 PCR,Ligation
Date:9/19 Ligation
Date:9/20 Enzyme inactivation,Ligation
Date:9/22 Transformation,Colony PCR,Electrophoresis,Miniprep
Date:9/23 Colony PCR,Electrophoresis,Streak
Date:9/25 Colony PCR

Creating parts of azurin

Date:8/16 Colony PCR,Ligation
Date:8/18 Colony PCR
Date:8/20 DNA extraction and purification of P.aeruginosa
Date:8/30 Electrophoresis,Refinement of DNA,PCR
Date:9/1 Electrophoresis
Date:9/3 Ligation,Transformation,PCR,Nanodrop