Team:KAIT Japan/Notebook

From 2012.igem.org

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The purified DNA
The purified DNA
::'''→Reflection:Band was less.'''
::'''→Reflection:Band was less.'''
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PCR Product
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===PCR Product===
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Revision as of 16:39, 2 September 2012

KAIT Japan2012 logo.png
KAIT Japan2012 Sub.png
Kaitjapan iGEM official.logo.png


Kaitjapan.home.png

Home

Kaitjapan project.png

Project

Kaitjapan parts.png

Parts

Kaitjapan protocol.png

Protocol

Kaitjapan notebook.png

Notebook

Kaitjapan results.png

Results

Kaitjapan safety.png

Safety

Kaitjapan human practice.png

Human Practice

Kaitjapan team.png

Team

Contents

Creating parts of Tar methylation region

Date:8/9

Colony PCR

→Reflection:We Took E.coli too many.You should take less E.coli.
→Reflection:The number of cycles was less.So,We increased The number of cycles in 8/11.(50 cycles)

Date:8/11

The purified DNA

→Reflection:Band was less.

PCR Product


Date:8/13

Confirmed of electrophoresis by PCR product and Ligation of the TA vector


Date:8/14

Transformation


Date:8/25

Miniprep

Creating parts of azurin

Date:8/16

Colony PCR Ligation


Date:8/18

Colony PCR


Date:8/20

DNA extraction and purification of P.aeruginosa