Team:KAIT Japan/Notebook

From 2012.igem.org

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='''Notebook'''=
='''Notebook'''=
:Our team pushed forward an experiment while making trial and error every day.
:Our team pushed forward an experiment while making trial and error every day.
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=='''Creating parts of Methylation'''==
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=='''Creating parts of Methylation and Azurin'''==
=='''Creating parts of Tar'''==
=='''Creating parts of Tar'''==
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:<big>'''Date:9/26'''</big> Liquid culture
:<big>'''Date:9/26'''</big> Liquid culture
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=='''Creating parts of Tar methylation region'''==
 
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:<big>'''Date:8/9'''</big> Colony PCR
 
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:<big>'''Date:8/11'''</big> The purified DNA
 
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:<big>'''Date:8/13'''</big> Confirmed of electrophoresis by PCR product and Ligation of the TA vector
 
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:<big>'''Date:8/14'''</big> Transformation
 
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:<big>'''Date:8/25'''</big> Miniprep
 
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:<big>'''Date:8/30'''</big> Electrophoresis,Refinement of DNA,PCR,Nanodrop
 
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:<big>'''Date:9/1'''</big> Miniprep,PCR,Elecrtophoresis
 
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:<big>'''Date:9/2'''</big> Meeting
 
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:<big>'''Date:9/5'''</big> Miniprep,Nanodrop
 
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:<big>'''Date:9/6'''</big> Miniprep,PCR,Electrophoresis
 
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:<big>'''Date:9/10'''</big> Colony PCR,Refinement of DNA,c,Miniprep
 
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:<big>'''Date:9/11'''</big> Primer design
 
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:<big>'''Date:9/12'''</big> Miniprep,Ligation
 
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:<big>'''Date:9/13'''</big> Electrophoresis,Refinement of DNA
 
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:<big>'''Date:9/14'''</big> Colony PCR
 
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:<big>'''Date:9/15'''</big> Ligation
 
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:<big>'''Date:9/16'''</big> PCR,Ligation
 
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:<big>'''Date:9/17'''</big> PCR,Ligation
 
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:<big>'''Date:9/19'''</big> Ligation
 
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:<big>'''Date:9/20'''</big> Enzyme inactivation,Ligation
 
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:<big>'''Date:9/22'''</big> Transformation,Colony PCR,Electrophoresis,Miniprep
 
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:<big>'''Date:9/23'''</big> Colony PCR,Electrophoresis,Streak
 
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:<big>'''Date:9/25'''</big> Colony PCR
 
|}
|}

Revision as of 18:26, 26 September 2012

KAIT Japan2012 logo.png
KAIT Japan2012 Sub.png
Kaitjapan iGEM official.logo.png


Kaitjapan.home.png

Home

Kaitjapan project.png

Project

Kaitjapan parts.png

Parts

Kaitjapan protocol.png

Protocol

Kaitjapan notebook.png

Notebook

Kaitjapan results.png

Results

Kaitjapan safety.png

Safety

Kaitjapan human practice.png

Human Practice

Kaitjapan team.png

Team

Notebook

Our team pushed forward an experiment while making trial and error every day.

Creating parts of Methylation and Azurin

Creating parts of Tar

Date:9/13 PCR and Colony PCR
Date:9/14 Electrophoretic confirmation
Date:9/15 Restriction enzyme processing
Date:9/16 Electrophoresis of the restriction enzyme processing product and Restriction enzyme processing
Date:9/17 Refinement of DNA and Ligation,Transformation
Date:9/18 Making of a master plate and Preculture,Recultivation
Date:9/19 Colony PCR and Electrophoresis
Date:9/20 Ligation and Transformation
Date:9/21 Inoculation
Date:9/22 Making of a master plate and Colony PCR,Electrophoresis,Preculture
Date:9/23 Electrophoretic confirmation and Electrophoresis of the PCR
Date:9/24 Miniprep and Electrophoresis
Date:9/25 PCR of the miniprep product and Electrophoresis

Creating parts of EnvZ

Date:9/10 Design of the primer and PCR,Elecrtophoresis,Miniprep
Date:9/13 PCR and Elecrtophoresis
Date:9/18 Design of the primer
Date:9/19 PCR and Elecrtophoresis
Date:9/20 Restriction enzyme processing
Date:9/21 Ligation and PCR,Elecrtophoresis
Date:9/23 PCR
Date:9/24 The refinement of DNA and Restriction enzyme processing,Ligation
Date:9/25 Transformation
Date:9/26 Liquid culture