Team:KAIT Japan/Notebook

From 2012.igem.org

(Difference between revisions)
(Creating parts of Tar methylation region)
(Creating parts of azurin)
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::Ligation
::Ligation
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:<big>'''Date:8/18'''</big>
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:<big>'''Date:8/18'''</big> Colony PCR
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::Colony PCR
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:<big>'''Date:8/20'''</big>
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:<big>'''Date:8/20'''</big> DNA extraction and purification of ''P.aeruginosa''
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::DNA extraction and purification of ''P.aeruginosa''
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Revision as of 10:30, 26 September 2012

KAIT Japan2012 logo.png
KAIT Japan2012 Sub.png
Kaitjapan iGEM official.logo.png


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Home

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Project

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Parts

Kaitjapan protocol.png

Protocol

Kaitjapan notebook.png

Notebook

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Results

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Safety

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Human Practice

Kaitjapan team.png

Team

Notebook

Our team pushed forward an experiment while making trial and error every day.

Creating parts of Tar methylation region

Date:8/9
Colony PCR
→Reflection:We Took E.coli too many.You should take less E.coli.
→Reflection:The number of cycles was less.So,We increased The number of cycles in 8/11.(50 cycles)
Date:8/11
The purified DNA
→Reflection:Band was less.
PCR Product
Date:8/13 Confirmed of electrophoresis by PCR product and Ligation of the TA vector
Date:8/14 Transformation
Date:8/25 Miniprep
Date:9/10
Date:9/11 Primer design
Date:9/12 Miniprep,Ligation
Date:9/13 Electrophoresis,Refinement of DNA
Date:9/14 Colony PCR
Date:9/15 Ligation
Date:9/16 PCR,Ligation
Date:9/17 PCR,Ligation
Date:9/19 Ligation
Date:9/20 Enzyme inactivation,Ligation
Date:9/22 Transformation,Colony PCR,Electrophoresis,Miniprep
Date:9/23 Colony PCR,Electrophoresis,Streak
Date:9/25 Colony PCR

Creating parts of azurin

Date:8/16
Colony PCR
Ligation
Date:8/18 Colony PCR
Date:8/20 DNA extraction and purification of P.aeruginosa