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Team:KAIST Korea/Notebook Labnote/2012 9
From 2012.igem.org
(Difference between revisions)
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<section id="1"> | <section id="1"> | ||
<div class="date">September 1<sup>st</sup> 2012</div></br> | <div class="date">September 1<sup>st</sup> 2012</div></br> | ||
| + | <div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:269px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:269px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br> | ||
| + | |||
| + | <div class="note-title">Bxb1_GTG_pBAD cloning with remaining DNAs</div> | ||
| + | |||
| + | <div id="content_note" > | ||
| + | |||
| + | <span id="little">Remaining Bxb1_GTG insert was cloned into pBADmycHisC vector. And electrotransformed into MG1655.</span> | ||
| + | </br></br> | ||
| + | |||
| + | </div> | ||
| + | |||
| + | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | ||
</section> | </section> | ||
<section id="2"> | <section id="2"> | ||
<div class="date">September 2<sup>nd</sup> 2012</div></br> | <div class="date">September 2<sup>nd</sup> 2012</div></br> | ||
| + | |||
| + | <div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:269px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:269px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br> | ||
| + | |||
| + | <div class="note-title">Bxb1_ATG_pTrc, pBAD, Bxb1_GTG_pTrc miniprep for double transformation, Bxb1_GTG_pBAD colony inoculation and vector miniprep NcoI single cut check.</div> | ||
| + | |||
| + | <div id="content_note" > | ||
| + | |||
| + | |||
| + | <b>Results</b></br></br> | ||
| + | |||
| + | <div align="center"><img id="figure" alt="2_0902Fig1" src="https://static.igem.org/mediawiki/2012/1/1c/KAIST_2_Sept.png"></img></div> | ||
| + | <div style="clear:both;"></div> | ||
| + | |||
| + | </br> | ||
| + | <b>Discussions</b></br></br> | ||
| + | <span id="little">All the vectors showed right sizes. But Bxb1_GTG_pBAD colony #1 through 3 have undesired bands. They may be the supercoiled vector.</span> | ||
| + | |||
| + | </br> | ||
| + | |||
| + | |||
| + | </div> | ||
| + | |||
| + | </br><div align="center"><img style="width:670px; height:2px;" src="https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png"></img></div></br> | ||
| + | |||
| + | <div class="note-title">Bxb1_ATG_pTrc, pBAD, Bxb1_GTG_pTrc double transformation into pPoC and pPoCpi</div> | ||
| + | |||
| + | |||
| + | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | ||
| + | |||
</section> | </section> | ||
<section id="3"> | <section id="3"> | ||
<div class="date">September 3<sup>rd</sup> 2012</div></br> | <div class="date">September 3<sup>rd</sup> 2012</div></br> | ||
| + | <div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:269px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:269px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br> | ||
| + | |||
| + | <div class="note-title">Bxb1 double transformants vector miniprep & PCR check</div> | ||
| + | |||
| + | <div id="content_note" > | ||
| + | |||
| + | |||
| + | |||
| + | <b>Results</b></br></br> | ||
| + | |||
| + | <div align="center"><img id="figure" alt="2_0903Fig1" src="https://static.igem.org/mediawiki/2012/1/18/KAIST_3_Sept.PNG"></img></div> | ||
| + | <div style="clear:both;"></div> | ||
| + | |||
| + | </br> | ||
| + | |||
| + | |||
| + | <b>Discussions</b></br></br> | ||
| + | <span id="little">Seemed to every colonies have appropriate vectors.</span> | ||
| + | |||
| + | </br> | ||
| + | |||
| + | |||
| + | </div> | ||
| + | |||
| + | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | ||
</section> | </section> | ||
<section id="4"> | <section id="4"> | ||
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<section id="10"> | <section id="10"> | ||
<div class="date">September 10<sup>th</sup> 2012</div></br> | <div class="date">September 10<sup>th</sup> 2012</div></br> | ||
| + | |||
| + | |||
| + | <div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:269px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:269px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br> | ||
| + | |||
| + | <div class="note-title">Bxb1_GTG_pBAD single cut check with different enzyme</div> | ||
| + | |||
| + | <div id="content_note" > | ||
| + | |||
| + | <span id="little">Single cut with SnaBI</span> | ||
| + | </br></br> | ||
| + | <b>Results</b></br></br> | ||
| + | |||
| + | <div align="center"><img id="figure" alt="2_0910Fig1" src="https://static.igem.org/mediawiki/2012/9/98/KAIST_10_Sept.PNG"></img></div> | ||
| + | <div style="clear:both;"></div> | ||
| + | |||
| + | </br> | ||
| + | |||
| + | <span id="little">No desired size(5.6kb) vector appeared.</span> | ||
| + | |||
| + | </br | ||
| + | |||
| + | |||
| + | </div> | ||
| + | |||
| + | |||
| + | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | ||
| + | |||
</section> | </section> | ||
<section id="11"> | <section id="11"> | ||
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<section id="12"> | <section id="12"> | ||
<div class="date">September 12<sup>th</sup> 2012</div></br> | <div class="date">September 12<sup>th</sup> 2012</div></br> | ||
| + | <div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:269px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:269px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br> | ||
| + | |||
| + | <div class="note-title">Bxb1_GTG_pBAD colony #4~7 inoculation, miniprep and single cut check | ||
| + | Single cut with SnaBI | ||
| + | </div> | ||
| + | |||
| + | <div id="content_note" > | ||
| + | |||
| + | <span id="little">Single cut with SnaBI</span> | ||
| + | </br></br> | ||
| + | <b>Results</b></br></br> | ||
| + | |||
| + | <div align="center"><img id="figure" alt="2_0912Fig1" src="https://static.igem.org/mediawiki/2012/1/13/KAIST_12_Sept.png"></img></div> | ||
| + | <div style="clear:both;"></div> | ||
| + | |||
| + | </br> | ||
| + | |||
| + | <span id="little">Cloning completed</span> | ||
| + | |||
| + | </br> | ||
| + | |||
| + | |||
| + | </div> | ||
| + | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | ||
| + | |||
| + | |||
</section> | </section> | ||
<section id="13"> | <section id="13"> | ||
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<section id="15"> | <section id="15"> | ||
<div class="date">September 15<sup>th</sup> 2012</div></br> | <div class="date">September 15<sup>th</sup> 2012</div></br> | ||
| + | <div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:269px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:269px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br> | ||
| + | |||
| + | <div class="note-title">BBa_C0060, BBa_C0061, BBa_C0062 transformation into TOP10</div> | ||
| + | |||
| + | <div id="content_note" > | ||
| + | |||
| + | <span id="little">Templates for Auto-regulated FlipFlop project.</span> | ||
| + | </br></br> | ||
| + | |||
| + | |||
| + | |||
| + | </div> | ||
| + | |||
| + | |||
| + | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | ||
</section> | </section> | ||
<section id="16"> | <section id="16"> | ||
<div class="date">September 16<sup>th</sup> 2012</div></br> | <div class="date">September 16<sup>th</sup> 2012</div></br> | ||
| + | <div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:269px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:269px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br> | ||
| + | |||
| + | <div class="note-title">BBa_C0060, BBa_C0061, BBa_C0062, bFMO inoculation and enzyme cut check</div> | ||
| + | |||
| + | <div id="content_note" > | ||
| + | |||
| + | <span id="little">BBa_C0060, BBa_C0061, BBa_C0062: from colony, EcoRI single cut<br><br> | ||
| + | <ul style="list-style-type:square;padding:0px 0px 0px 40px"> | ||
| + | <li> BBa_C0060: 2859bp </li> | ||
| + | <li> BBa_C0061: 2688bp </li> | ||
| + | <li> BBa_C0062: 2826bp </li> | ||
| + | <li>bFMO: from cell stock, EcoRI single cut</li> | ||
| + | </ul></span> | ||
| + | </br></br> | ||
| + | <b>Results</b></br></br> | ||
| + | |||
| + | <div align="center"><img id="figure" alt="2_0916#Fig#1" src="https://static.igem.org/mediawiki/2012/0/0f/16_Sept.JPG"></img></div> | ||
| + | <div style="clear:both;"></div> | ||
| + | |||
| + | |||
| + | |||
| + | </br> | ||
| + | |||
| + | |||
| + | </div> | ||
| + | |||
| + | |||
| + | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | ||
| + | |||
</section> | </section> | ||
<section id="17"> | <section id="17"> | ||
<div class="date">September 17<sup>th</sup> 2012</div></br> | <div class="date">September 17<sup>th</sup> 2012</div></br> | ||
| + | |||
| + | <div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:269px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img stylsght:3px;width:269px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br> | ||
| + | |||
| + | <div class="note-title">Template preparation for OE PCR</div> | ||
| + | |||
| + | <div id="content_note" > | ||
| + | </br> | ||
| + | <span id="little">Promoter construct: 210bp</span> | ||
| + | </br> | ||
| + | <b>Results</b></br></br> | ||
| + | |||
| + | <div align="center"><img id="figure" alt="2_0917Fig1" src="https://static.igem.org/mediawiki/2012/d/d3/KAIST_17_Sept.JPG"></img></div> | ||
| + | <div style="clear:both;"></div> | ||
| + | <span id="little">Promoter PCR failed</span> | ||
| + | </br> | ||
| + | <div align="center"><img id="figure" alt="2_0917Fig2" src="https://static.igem.org/mediawiki/2012/d/d7/KAIST_17_Sept_2.JPG"></img> | ||
| + | |||
| + | |||
| + | |||
| + | </div> | ||
| + | |||
| + | |||
| + | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | ||
</section> | </section> | ||
<section id="18"> | <section id="18"> | ||
<div class="date">September 18<sup>th</sup> 2012</div></br> | <div class="date">September 18<sup>th</sup> 2012</div></br> | ||
| + | <div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:269px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img stylsght:3px;width:269px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br> | ||
| + | |||
| + | <div class="note-title">Template preparation for OE PCR</div> | ||
| + | |||
| + | <div id="content_note" > | ||
| + | |||
| + | |||
| + | <b>Results</b></br></br> | ||
| + | |||
| + | <div align="center"><img id="figure" alt="2_0918Fig1" src="https://static.igem.org/mediawiki/2012/5/54/KAIST_18_Sept.JPG"></img></div> | ||
| + | <div style="clear:both;"></div> | ||
| + | |||
| + | </br> | ||
| + | |||
| + | <span id="little">Terminator PCR failed</span> | ||
| + | |||
| + | </br> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | </div> | ||
| + | |||
| + | |||
| + | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | ||
| + | |||
</section> | </section> | ||
<section id="19"> | <section id="19"> | ||
<div class="date">September 19<sup>th</sup> 2012</div></br> | <div class="date">September 19<sup>th</sup> 2012</div></br> | ||
| + | <div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:269px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img stylsght:3px;width:269px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br> | ||
| + | |||
| + | <div class="note-title">Template preparation for OE PCR</div> | ||
| + | |||
| + | <div id="content_note" > | ||
| + | |||
| + | <span id="little">Terminator: 179bp</span> | ||
| + | </br></br> | ||
| + | <b>Results</b></br></br> | ||
| + | |||
| + | <div align="center"><img id="figure" alt="2_0919Fig1" src="https://static.igem.org/mediawiki/2012/4/44/KAIST_19_Sept.JPG"></img></div> | ||
| + | <div style="clear:both;"></div> | ||
| + | |||
| + | </br> | ||
| + | |||
| + | <span id="little"> → Gel extracted</span> | ||
| + | |||
| + | </br> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | </div> | ||
| + | |||
| + | |||
| + | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | ||
| + | |||
</section> | </section> | ||
<section id="20"> | <section id="20"> | ||
<div class="date">September 20<sup>th</sup> 2012</div></br> | <div class="date">September 20<sup>th</sup> 2012</div></br> | ||
| + | <div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:269px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img stylsght:3px;width:269px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br> | ||
| + | |||
| + | <div class="note-title">Template preparation for OE PCR</div> | ||
| + | |||
| + | <div id="content_note" > | ||
| + | |||
| + | <span id="little">Bxb1 Xis: 777bp</span> | ||
| + | </br></br> | ||
| + | <b>Results</b></br></br> | ||
| + | |||
| + | <div align="center"><img id="figure" alt="2_0920Fig1" src="https://static.igem.org/mediawiki/2012/8/8e/KAIST_20_Sept.JPG"></img></div> | ||
| + | <div style="clear:both;"></div> | ||
| + | <span id="little">bFMO PCR w/ various condtions</span> | ||
| + | |||
| + | </br> | ||
| + | <div align="center"><img id="figure" alt="2_0920Fig2" src="https://static.igem.org/mediawiki/2012/c/cd/KAIST_20_Sept_2.JPG"></img></div> | ||
| + | <div style="clear:both;"></div> | ||
| + | |||
| + | </br> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | </div> | ||
| + | |||
| + | </br><div align="center"><img style="width:670px; height:2px;" src="https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png"></img></div></br> | ||
| + | |||
| + | <div class="note-title">Bxb1_Xis-pGEM_B1 transfomation</div> | ||
| + | |||
| + | <div id="content_note" > | ||
| + | |||
| + | <span id="little">Synthesized gene – Bxb1_Xis arrived and transformed into TOP10</span> | ||
| + | </br></br> | ||
| + | |||
| + | </div> | ||
| + | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | ||
| + | |||
</section> | </section> | ||
<section id="21"> | <section id="21"> | ||
| Line 249: | Line 526: | ||
<section id="22"> | <section id="22"> | ||
<div class="date">September 22<sup>nd</sup> 2012</div></br> | <div class="date">September 22<sup>nd</sup> 2012</div></br> | ||
| + | <div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:269px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img stylsght:3px;width:269px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br> | ||
| + | |||
| + | <div class="note-title">Overlapping extension PCR</div> | ||
| + | |||
| + | <div id="content_note" > | ||
| + | |||
| + | |||
| + | <b>Results</b></br></br> | ||
| + | |||
| + | <div align="center"><img id="figure" alt="2_0922Fig1" src="https://static.igem.org/mediawiki/2012/f/f5/KAIST_22_Sept.JPG"></img></div> | ||
| + | <div style="clear:both;"></div> | ||
| + | |||
| + | </br> | ||
| + | |||
| + | <span id="little"> → Gel extracted</span> | ||
| + | |||
| + | </br> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | </div> | ||
| + | |||
| + | |||
| + | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | ||
</section> | </section> | ||
<section id="23"> | <section id="23"> | ||
<div class="date">September 23<sup>rd</sup> 2012</div></br> | <div class="date">September 23<sup>rd</sup> 2012</div></br> | ||
| + | <div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:269px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img stylsght:3px;width:269px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br> | ||
| + | |||
| + | <div class="note-title">Overlapping extension PCR-Nested PCR</div> | ||
| + | |||
| + | <div id="content_note" > | ||
| + | |||
| + | |||
| + | <b>Results</b></br></br> | ||
| + | |||
| + | <div align="center"><img id="figure" alt="2_0923Fig1" src="https://static.igem.org/mediawiki/2012/4/41/KAIST_23_Sept.JPG"></img></div> | ||
| + | <div style="clear:both;"></div> | ||
| + | |||
| + | </br> | ||
| + | |||
| + | |||
| + | |||
| + | <b>Discussions</b></br></br> | ||
| + | <span id="little">There was too little full construct nested. We think this is due to primer interfering by its LVA homology.</span> | ||
| + | |||
| + | </br> | ||
| + | |||
| + | |||
| + | </div> | ||
| + | |||
| + | </br><div align="center"><img style="width:670px; height:2px;" src="https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png"></img></div></br> | ||
| + | |||
| + | <div class="note-title">Overlapping extension PCR 2</div> | ||
| + | |||
| + | <div id="content_note" > | ||
| + | |||
| + | |||
| + | <b>Results</b></br></br> | ||
| + | |||
| + | <div align="center"><img id="figure" alt="2_0923Fig2" src="https://static.igem.org/mediawiki/2012/a/ae/KAIST_23_Sept_2.JPG"></img></div> | ||
| + | <div style="clear:both;"></div> | ||
| + | |||
| + | </br> | ||
| + | <b>Discussions</b></br></br> | ||
| + | <span id="little">Bands were more intense than those of first trial.</span> | ||
| + | |||
| + | </br> | ||
| + | </div> | ||
| + | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | ||
</section> | </section> | ||
<section id="24"> | <section id="24"> | ||
<div class="date">September 24<sup>th</sup> 2012</div></br> | <div class="date">September 24<sup>th</sup> 2012</div></br> | ||
| + | <div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:269px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img stylsght:3px;width:269px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br> | ||
| + | |||
| + | <div class="note-title">Overlapping extension PCR Gel Extraction</div> | ||
| + | |||
| + | <div id="content_note" > | ||
| + | |||
| + | |||
| + | <b>Results</b></br></br> | ||
| + | |||
| + | <div align="center"><img id="figure" alt="2_0924Fig1" src="https://static.igem.org/mediawiki/2012/6/6e/KAIST_24_Sept.JPG"></img></div> | ||
| + | <div style="clear:both;"></div> | ||
| + | |||
| + | </br> | ||
| + | |||
| + | |||
| + | |||
| + | </div> | ||
| + | |||
| + | </br><div align="center"><img style="width:670px; height:2px;" src="https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png"></img></div></br> | ||
| + | |||
| + | |||
| + | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | ||
| + | |||
</section> | </section> | ||
<section id="25"> | <section id="25"> | ||
| Line 288: | Line 656: | ||
<!-- | <!-- | ||
##########SAMPLE!!!########## | ##########SAMPLE!!!########## | ||
| + | <div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:269px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img stylsght:3px;width:269px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br> | ||
| + | |||
<div class="note-title">TITLE</div> | <div class="note-title">TITLE</div> | ||
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<b>Results</b></br></br> | <b>Results</b></br></br> | ||
| - | <div align="center"><img id="figure" alt=" | + | <div align="center"><img id="figure" alt="2_09##Fig#" src="#"></img></div> |
<div style="clear:both;"></div> | <div style="clear:both;"></div> | ||
</br> | </br> | ||
| - | <span id="little"></span> | + | <span id="little">CONTENT</span> |
| + | |||
| + | </br> | ||
| + | |||
| + | <b>Discussions</b></br></br> | ||
| + | <span id="little">CONTENT</span> | ||
</br> | </br> | ||
| Line 308: | Line 683: | ||
</div> | </div> | ||
| - | </br>< | + | </br><div align="center"><img style="width:670px; height:2px;" src="https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png"></img></div></br> |
<div class="note-title">TITLE2</div> | <div class="note-title">TITLE2</div> | ||
| Line 318: | Line 693: | ||
<b>Results</b></br></br> | <b>Results</b></br></br> | ||
| - | <div align="center"><img id="figure" alt=" | + | <div align="center"><img id="figure" alt="2_09##Fig#" src="#"></img></div> |
<div style="clear:both;"></div> | <div style="clear:both;"></div> | ||
</br> | </br> | ||
| - | <span id="little"></span> | + | <span id="little">CONTENT</span> |
</br> | </br> | ||
</div> | </div> | ||
| - | |||
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | ||
| - | + | <ul style="list-style-type:square;padding:0px 0px 0px 40px"> | |
| + | <li> </li> | ||
| + | <li> </li> | ||
| + | <li> </li> | ||
| + | </ul> | ||
--> | --> | ||
Revision as of 00:42, 27 September 2012
2012 KAIST Korea
Mail : kaist.igem.2012@gmail.com
Twitter : twitter.com/KAIST_iGEM_2012
Facebook : www.facebook.com/KAISTiGEM2012
Notebook : Labnote-September
Labnote
SeptemberSeptember 1st 2012
Flip FlopBxb1_GTG_pBAD cloning with remaining DNAs
Remaining Bxb1_GTG insert was cloned into pBADmycHisC vector. And electrotransformed into MG1655.
Back to the Calendar
Back to the CalendarSeptember 2nd 2012
Flip FlopBxb1_ATG_pTrc, pBAD, Bxb1_GTG_pTrc miniprep for double transformation, Bxb1_GTG_pBAD colony inoculation and vector miniprep NcoI single cut check.
Results
Discussions
All the vectors showed right sizes. But Bxb1_GTG_pBAD colony #1 through 3 have undesired bands. They may be the supercoiled vector.
Bxb1_ATG_pTrc, pBAD, Bxb1_GTG_pTrc double transformation into pPoC and pPoCpi
Back to the Calendar
September 3rd 2012
Flip FlopBxb1 double transformants vector miniprep & PCR check
Results
Discussions
Seemed to every colonies have appropriate vectors.
Back to the Calendar
September 4th 2012
September 5th 2012
September 6th 2012
September 7th 2012
September 8th 2012
September 9th 2012
September 10th 2012
Flip FlopBxb1_GTG_pBAD single cut check with different enzyme
Single cut with SnaBI
Results
No desired size(5.6kb) vector appeared.
Back to the Calendar
Flip Flop
Flip Flop
Flip Flop
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September 11th 2012
September 12th 2012
Flip FlopBxb1_GTG_pBAD colony #4~7 inoculation, miniprep and single cut check
Single cut with SnaBI
Single cut with SnaBI
Results
Cloning completed
Back to the Calendar
September 13th 2012
September 14th 2012
September 15th 2012
Flip FlopBBa_C0060, BBa_C0061, BBa_C0062 transformation into TOP10
Templates for Auto-regulated FlipFlop project.
Back to the Calendar
September 16th 2012
Flip FlopBBa_C0060, BBa_C0061, BBa_C0062, bFMO inoculation and enzyme cut check
BBa_C0060, BBa_C0061, BBa_C0062: from colony, EcoRI single cut
Back to the Calendar
- BBa_C0060: 2859bp
- BBa_C0061: 2688bp
- BBa_C0062: 2826bp
- bFMO: from cell stock, EcoRI single cut
September 17th 2012
Template preparation for OE PCR
Promoter construct: 210bp
Results
Promoter PCR failed
Back to the Calendar
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Back to the Calendar
September 18th 2012
Template preparation for OE PCR
Results
Terminator PCR failed
Back to the Calendar
September 19th 2012
Template preparation for OE PCR
Terminator: 179bp
Results
→ Gel extracted
Back to the Calendar
September 20th 2012
Template preparation for OE PCR
Bxb1 Xis: 777bp
Results
bFMO PCR w/ various condtions
Bxb1_Xis-pGEM_B1 transfomation
Synthesized gene – Bxb1_Xis arrived and transformed into TOP10
Back to the Calendar
September 21st 2012
September 22nd 2012
Overlapping extension PCR
Results
→ Gel extracted
Back to the Calendar
September 23rd 2012
Overlapping extension PCR-Nested PCR
Results
Discussions
There was too little full construct nested. We think this is due to primer interfering by its LVA homology.
Overlapping extension PCR 2
Results
Discussions
Bands were more intense than those of first trial.
Back to the Calendar
September 24th 2012
Overlapping extension PCR Gel Extraction
Results
September 25th 2012
September 26th 2012
September 27th 2012
September 28th 2012
September 29th 2012
September 30th 2012
September 31st 2012
