Team:HokkaidoU Japan/Notebook/plastic Week 8
From 2012.igem.org
August 20th
Digestion
We digested three samples.
Digestions of PhaC and pSB1C3 with XbaI and SpeI.
DNA solution PhaC | 12 ul |
XbaI | 1 ul |
SpeI | 1 ul |
10xM buffer | 2 ul |
DW | 4 ul |
Total | 20 ul |
Digestion of PhaA with XbaI site and SpeI site.
DNA solution PhaA | 7 ul |
XbaI | 1 ul |
SpeI | 1 ul |
10xM buffer | 2 ul |
DW | 9 ul |
Total | 20 ul |
And digestion of PhaB with XbaI site and SpeI site.
DNA solution PhaB | 7 ul |
XbaI | 1 ul |
SpeI | 1 ul |
10xM buffer | 2 ul |
DW | 9 ul |
Total | 20 ul |
August 21st
August 22nd
August 24th
August 25th
Colony PCR
The length of PhaA on pSB1C3 and PhaB on pSB1C3 were confirmed by colony PCR.
The result showed PhaA and pSB1C3 were ligated correctly.
Liquid culture
Cultivation of bacteria holds RBS (B0034) was started.
Digestion
Digestion to divide PhaC(BBa_K342001) with XbaI and SpeI. The result shows that the digestion succeeded.