Team:HokkaidoU Japan/Notebook/plastic Week 7
From 2012.igem.org
(Difference between revisions)
(→PCR) |
(→August 17nd) |
||
Line 45: | Line 45: | ||
</p> | </p> | ||
- | + | </div> | |
+ | |||
+ | <div> | ||
+ | ===Electrophoresis=== | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | |||
+ | <div> | ||
+ | ===Gel extraction=== | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
Revision as of 13:41, 26 August 2012
Contents |
August 17nd
PCR
A PCR of pGEM to get linear PhaA and PhaB using Laury primer and X-PhaA-up-F, S-PhaA-dw-R, X-PhaB-up-F, S-PhaB-dw-R.
pGEM(pGEM 1ul + DW 9ul) | 1 ul |
up primer(10mM) | 1 ul |
down primer(10mM) | 1 ul |
25mM MgSO4 | 3 ul |
2mM sNTPs | 5 ul |
KOD plus neo | 1ul |
10xPCR buffer | 5 ul |
DW | 33 ul |
PCR:60sec, 30 cycle 1h8m
Electrophoresis
Gel extraction