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Team:HokkaidoU Japan/Notebook/plastic Week 7
From 2012.igem.org
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Revision as of 13:06, 26 August 2012
August 17nd
PCR
A PCR of pGEM to get linear Pha A and PhaB using Laury primer.
| pGEM(pGEM 1ul + DW 9ul) | 1 ul |
| up primer(10mM) | 1 ul |
| down primer(10mM) | 1 ul |
| 1 ul | |
| 25mM MgSO4 | 3 ul |
| 2mM sNTPs | 5 ul |
| 10xPCR buffer | 5 ul |
| DW | 33 ul |
PCR:60sec, 30 cycle 1h8m
