"
Team:HokkaidoU Japan/Notebook/plastic Week 7
From 2012.igem.org
(Difference between revisions)
(→PCR) |
(→PCR) |
||
| Line 22: | Line 22: | ||
|- | |- | ||
|KOD plus Neo | |KOD plus Neo | ||
| - | + | |1 ul | |
|- | |- | ||
|25mM MgSO4 | |25mM MgSO4 | ||
| - | | | + | |3 ul |
|- | |- | ||
|2mM sNTPs | |2mM sNTPs | ||
| - | | | + | |5 ul |
|- | |- | ||
|10xPCR buffer | |10xPCR buffer | ||
Revision as of 13:05, 26 August 2012
August 17nd
PCR
A PCR of pGEM to get linear Pha A and PhaB using Laury primer.
| pGEM(pGEM 1ul + DW 9ul) | 1 ul |
| up primer(10mM) | 1 ul |
| down primer(10mM) | 1 ul |
| 1 ul | |
| 25mM MgSO4 | 3 ul |
| 2mM sNTPs | 5 ul |
| 10xPCR buffer | 5 ul |
| DW | 33 ul |
PCR:60sec, 30 cycle 1h8m
