Team:HokkaidoU Japan/Notebook/plastic Week 7

From 2012.igem.org

(Difference between revisions)
(→Anplify of DNA)
(→PCR)
Line 8: Line 8:
===PCR===
===PCR===
<p>
<p>
-
PCR of pGEM to get linear Pha A and PhaB with primer.  
+
A PCR of pGEM to get linear Pha A and PhaB using Laury primer.  
{|class="hokkaidou-table-digestion"
{|class="hokkaidou-table-digestion"
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   |1 ul
   |1 ul
   |-
   |-
-
   |
+
   |down primer(10mM)
   |1 ul
   |1 ul
   |-
   |-
-
   |10xM buffer
+
　|KOD plus Neo
-
   |2 ul
+
　|1ul
 +
  |-
 +
  |25mM MgSO4
 +
  |3ul
 +
  |-
 +
  |2mM sNTPs
 +
  |5ul
 +
  |-
 +
   |10xPCR buffer
 +
   |5 ul
   |-
   |-
   |DW
   |DW
-
   |4 ul
+
   |33 ul
   |-
   |-
-
  |Total
 
-
  |20 ul
 
   |}
   |}
 +
 +
PCR:60sec, 30 cycle 1h8m

Revision as of 13:03, 26 August 2012

Retrieved from "http://2012.igem.org/Team:HokkaidoU_Japan/Notebook/plastic_Week_7"