Team:HokkaidoU Japan/Notebook/plastic Week 7

From 2012.igem.org

(Difference between revisions)
(→Anplify of DNA)
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==August  17nd==
==August  17nd==
<div>
<div>
-
===Anplify of DNA===
+
===PCR===
<p>
<p>
 +
PCR of pGEM to get linear Pha A and PhaB with primer.
 +
 +
{|class="hokkaidou-table-digestion"
 +
  |-
 +
  |pGEM(pGEM 1ul + DW 9ul)
 +
  |1 ul
 +
  |-
 +
  |up primer(10mM)
 +
  |1 ul
 +
  |-
 +
  |
 +
  |1 ul
 +
  |-
 +
  |10xM buffer
 +
  |2 ul
 +
  |-
 +
  |DW
 +
  |4 ul
 +
  |-
 +
  |Total
 +
  |20 ul
 +
  |}
 +
 +
</p>
</p>

Revision as of 12:53, 26 August 2012

Retrieved from "http://2012.igem.org/Team:HokkaidoU_Japan/Notebook/plastic_Week_7"