"
Team:HokkaidoU Japan/Notebook/plastic Week 6
From 2012.igem.org
(Difference between revisions)
| (One intermediate revision not shown) | |||
| Line 3: | Line 3: | ||
<div id="hokkaidou-column-main"> | <div id="hokkaidou-column-main"> | ||
<!-- DO NOT EDIT OVER THIS LINE @iTakeshi --> | <!-- DO NOT EDIT OVER THIS LINE @iTakeshi --> | ||
| - | |||
<div class="hokkaidou-notebook-daily"> | <div class="hokkaidou-notebook-daily"> | ||
| - | ==August 6th== | + | ===August 6th=== |
| - | <div> | + | <div class="hokkaidou-section"> |
| - | ==Polymer extract== | + | ====Polymer extract==== |
| - | + | ||
@Taguchi lab | @Taguchi lab | ||
| - | |||
#sample1 JM109 culture from July 18th 1.4643 g , 0.0054 (tube+polymer , polymer) | #sample1 JM109 culture from July 18th 1.4643 g , 0.0054 (tube+polymer , polymer) | ||
#sample2 JM109 culture from July 18th 1.4642 g , 0.0040 | #sample2 JM109 culture from July 18th 1.4642 g , 0.0040 | ||
#sample3 DH5a 1.4666 g , 0.0056 | #sample3 DH5a 1.4666 g , 0.0056 | ||
| - | ==Preculture== | + | ====Preculture==== |
| - | + | ||
Inoculated from three different plates (Name it 1,2,3).<br /> | Inoculated from three different plates (Name it 1,2,3).<br /> | ||
Cultured in media of LB 150 ul + 1.5 ul of Amp.<br /> | Cultured in media of LB 150 ul + 1.5 ul of Amp.<br /> | ||
30C August 6th 20:00 ~ 7th 10:00<br /> | 30C August 6th 20:00 ~ 7th 10:00<br /> | ||
| - | |||
| - | |||
| + | <br style="line-height: 0; clear: both;" /> | ||
| + | </div></div> | ||
<div class="hokkaidou-notebook-daily"> | <div class="hokkaidou-notebook-daily"> | ||
| - | + | ===August 7th=== | |
| - | ==August 7th== | + | <div class="hokkaidou-section"> |
| - | <div> | + | ====Liquid culture==== |
| - | ==Liquid culture== | + | |
| - | + | ||
| - | + | ||
We made 8 samples to see if Panthotenic acid is necessary for plastic growth. <br /> | We made 8 samples to see if Panthotenic acid is necessary for plastic growth. <br /> | ||
| - | + | *Panthotenic acid (+)1.5 ml 4 samples | |
| - | + | *Panthotenic acid (+)0.7 ml 2 samples | |
| - | Panthotenic acid (+)1.5 ml 4 samples | + | *Panthotenic acid (-)0.7 ml 2 samples |
| - | Panthotenic acid (+)0.7 ml 2 samples | + | |
| - | Panthotenic acid (-)0.7 ml 2 samples | + | |
Incubated liquid culture from August 7th 11:00 until 9th 09:50.<br /> | Incubated liquid culture from August 7th 11:00 until 9th 09:50.<br /> | ||
| - | </ | + | <br style="line-height: 0; clear: both;" /> |
</div></div> | </div></div> | ||
| - | |||
<div class="hokkaidou-notebook-daily"> | <div class="hokkaidou-notebook-daily"> | ||
| - | + | ===August 9th=== | |
| - | ==August 9th== | + | <div class="hokkaidou-section"> |
| - | <div> | + | |
@Taguchi lab | @Taguchi lab | ||
| - | ==Harvest== | + | ====Harvest==== |
| - | + | ||
We did the protocol of harvest on 8 samples. | We did the protocol of harvest on 8 samples. | ||
| - | |||
| - | |||
| + | <br style="line-height: 0; clear: both;" /> | ||
| + | </div></div> | ||
<div class="hokkaidou-notebook-daily"> | <div class="hokkaidou-notebook-daily"> | ||
| - | + | ===August 10th=== | |
| - | ==August 10th== | + | <div class="hokkaidou-section"> |
| - | <div> | + | ====Plasmid extraction==== |
| - | ==Plasmid extraction== | + | |
| - | + | ||
The plasmid of BBa_K342001(PhaC) was extracted by FastGene Plasmid Mini Kit(Nippon Genetics) and we got 50 ul of DNA solutions. | The plasmid of BBa_K342001(PhaC) was extracted by FastGene Plasmid Mini Kit(Nippon Genetics) and we got 50 ul of DNA solutions. | ||
| - | </ | + | |
| + | <br style="line-height: 0; clear: both;" /> | ||
</div></div> | </div></div> | ||
| - | |||
<div class="hokkaidou-notebook-daily"> | <div class="hokkaidou-notebook-daily"> | ||
| - | + | ===August 11th=== | |
| - | ==August 11th== | + | <div class="hokkaidou-section"> |
| - | <div> | + | ====Sequencing==== |
| - | ==Sequencing== | + | |
| - | + | ||
BBa_K342001(PhaC) was seqenced.<br /> | BBa_K342001(PhaC) was seqenced.<br /> | ||
| - | + | <br style="line-height: 0; clear: both;" /> | |
| - | </ | + | </div></div> |
| - | </div><div> | + | |
<!-- DO NOT EDIT UNDER THIS LINE @iTakeshi --> | <!-- DO NOT EDIT UNDER THIS LINE @iTakeshi --> | ||
Latest revision as of 02:58, 27 September 2012
Contents |
August 6th
Polymer extract
@Taguchi lab
- sample1 JM109 culture from July 18th 1.4643 g , 0.0054 (tube+polymer , polymer)
- sample2 JM109 culture from July 18th 1.4642 g , 0.0040
- sample3 DH5a 1.4666 g , 0.0056
Preculture
Inoculated from three different plates (Name it 1,2,3).
Cultured in media of LB 150 ul + 1.5 ul of Amp.
30C August 6th 20:00 ~ 7th 10:00
August 7th
Liquid culture
We made 8 samples to see if Panthotenic acid is necessary for plastic growth.
- Panthotenic acid (+)1.5 ml 4 samples
- Panthotenic acid (+)0.7 ml 2 samples
- Panthotenic acid (-)0.7 ml 2 samples
Incubated liquid culture from August 7th 11:00 until 9th 09:50.
August 9th
@Taguchi lab
Harvest
We did the protocol of harvest on 8 samples.
August 10th
Plasmid extraction
The plasmid of BBa_K342001(PhaC) was extracted by FastGene Plasmid Mini Kit(Nippon Genetics) and we got 50 ul of DNA solutions.
August 11th
Sequencing
BBa_K342001(PhaC) was seqenced.
