Team:HokkaidoU Japan/Notebook/plastic Week 6

From 2012.igem.org

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===August 6th===
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<div class="hokkaidou-section">
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====Polymer extract====
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@Taguchi lab
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#sample1 JM109 culture from July 18th 1.4643 g , 0.0054 (tube+polymer , polymer)
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#sample2 JM109 culture from July 18th 1.4642 g , 0.0040
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#sample3 DH5a                        1.4666 g , 0.0056
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====Preculture====
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Inoculated from three different plates (Name it 1,2,3).<br />
 +
Cultured in media of LB 150 ul + 1.5 ul of Amp.<br />
 +
30C  August 6th 20:00 ~ 7th 10:00<br />
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<br style="line-height: 0; clear: both;" />
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</div></div>
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<div class="hokkaidou-notebook-daily">
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===August 7th===
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<div class="hokkaidou-section">
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====Liquid culture====
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We made 8 samples to see if Panthotenic acid is necessary for plastic growth. <br />
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*Panthotenic acid (+)1.5 ml 4 samples
 +
*Panthotenic acid (+)0.7 ml 2 samples
 +
*Panthotenic acid (-)0.7 ml 2 samples
 +
 +
Incubated liquid culture from August 7th 11:00 until 9th 09:50.<br />
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<br style="line-height: 0; clear: both;" />
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</div></div>
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<div class="hokkaidou-notebook-daily">
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===August 9th===
 +
<div class="hokkaidou-section">
 +
@Taguchi lab
 +
====Harvest====
 +
We did the protocol of harvest on 8 samples.
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 +
<br style="line-height: 0; clear: both;" />
 +
</div></div>
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<div class="hokkaidou-notebook-daily">
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===August 10th===
 +
<div class="hokkaidou-section">
 +
====Plasmid extraction====
 +
The plasmid of BBa_K342001(PhaC) was extracted by FastGene Plasmid Mini Kit(Nippon Genetics) and we got 50 ul of DNA solutions.
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<br style="line-height: 0; clear: both;" />
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</div></div>
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<div class="hokkaidou-notebook-daily">
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===August 11th===
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<div class="hokkaidou-section">
 +
====Sequencing====
 +
BBa_K342001(PhaC) was seqenced.<br />
 +
 +
<br style="line-height: 0; clear: both;" />
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</div></div>
<!-- DO NOT EDIT UNDER THIS LINE @iTakeshi -->
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Latest revision as of 02:58, 27 September 2012

Contents

August 6th

Polymer extract

@Taguchi lab

  1. sample1 JM109 culture from July 18th 1.4643 g , 0.0054 (tube+polymer , polymer)
  2. sample2 JM109 culture from July 18th 1.4642 g , 0.0040
  3. sample3 DH5a 1.4666 g , 0.0056

Preculture

Inoculated from three different plates (Name it 1,2,3).
Cultured in media of LB 150 ul + 1.5 ul of Amp.
30C August 6th 20:00 ~ 7th 10:00


August 7th

Liquid culture

We made 8 samples to see if Panthotenic acid is necessary for plastic growth.

  • Panthotenic acid (+)1.5 ml 4 samples
  • Panthotenic acid (+)0.7 ml 2 samples
  • Panthotenic acid (-)0.7 ml 2 samples

Incubated liquid culture from August 7th 11:00 until 9th 09:50.


August 9th

@Taguchi lab

Harvest

We did the protocol of harvest on 8 samples.


August 10th

Plasmid extraction

The plasmid of BBa_K342001(PhaC) was extracted by FastGene Plasmid Mini Kit(Nippon Genetics) and we got 50 ul of DNA solutions.


August 11th

Sequencing

BBa_K342001(PhaC) was seqenced.



Retrieved from "http://2012.igem.org/Team:HokkaidoU_Japan/Notebook/plastic_Week_6"