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Team:HokkaidoU Japan/Notebook/plastic Week 5
From 2012.igem.org
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==August 2nd== | ==August 2nd== | ||
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==Transformation== | ==Transformation== | ||
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| + | ==August 3rd== | ||
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| + | ==Liquid culture== | ||
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| + | Liquid culture of Transformed BL21(DE3) | ||
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{{Team:HokkaidoU_Japan/footer}} | {{Team:HokkaidoU_Japan/footer}} | ||
Revision as of 05:18, 3 August 2012
Contents |
August 2nd
Transformation
Transformation of pGEM into BL21. This transformation is the plastic production test in BL21.
- Added 1ul of plasmid DNA to 50ul of thawed competent cells on ice.
- Incubated on ice for 30min.
- Added 600ul of LB.
- Prepared and Labeled two petri dishes with LBA.
- Plate 300ul of the transformation onto LBA dish and spread.
- Added 900ul of LB to 100ul of the transformation and plated 300ul of it onto LBA dish then spread.
- Incubated the plates at 37C for OOhrs.
August 3rd
Liquid culture
Liquid culture of Transformed BL21(DE3)
