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Team:HokkaidoU Japan/Notebook/plastic Week 11
From 2012.igem.org
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RBS on pSB1A2 was ligated with PhaA and PhaB. | RBS on pSB1A2 was ligated with PhaA and PhaB. | ||
Revision as of 10:04, 10 September 2012
Contents |
September 10th
PCR
PhaA was multiplied from pGEM with XbaI and SpeI restriction sites by PCR.
Digestion
PhaA was digested with XbaI and SpeI. RBS on pSB1A2 was digested with SpeI.
Gel extraction
We confirmed succession of digestion by electrophoresis.
And then DNA were extracted from TBE gel.
Ligation
RBS on pSB1A2 was ligated with PhaA and PhaB.
Transformation
These ligated DNAs transformed into E.coli (strain: DH5α).
And then we spread fungus liquid added LB on plates.
