Team:HokkaidoU Japan/Notebook/overall protocols
From 2012.igem.org
Transformation
- Add 1~2 ul of DNA to 50 ul of thawed competent cells on ice.
- Incubate on ice for 30 min.
- Add 600 ul of LB.
- Incubate the cells for 2 hours at 37C if added DNA plasmid have antibiotic resistance other than ampicillin.
- Prepare and Label two plastic plates with LB and appropriate antibiotic.
- Plate 300 ul of the culture onto first dish and spread.
- Add 900 ul of LB to 100 ul of the culture and plate 300 ul of it onto second dish and spread.
- Incubate the plates at 37C for 16~20 hours.