"
Team:HokkaidoU Japan/Notebook
From 2012.igem.org
(Difference between revisions)
| Line 27: | Line 27: | ||
**Cultivation on LBA(B0015,RBS) and LBC(Ag43, pLacI-RBS-Ag43) | **Cultivation on LBA(B0015,RBS) and LBC(Ag43, pLacI-RBS-Ag43) | ||
==5th== | ==5th== | ||
| + | Transformation | ||
| + | *Transformation of K346007(Ag43)in DH5α | ||
| + | **Cultivation on LBC. | ||
| + | |||
Single colony isolation | Single colony isolation | ||
*Single colony isolation of parts written above. | *Single colony isolation of parts written above. | ||
Revision as of 08:11, 6 July 2012
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
|---|
Contents |
Hello
We are team HokkaidoU Japan! Today we learn and start to edit wiki.
(>ω<)
Dear Mr.Ortiz, I saw the help page which you edited.
hola!
July
4th
Transformation
- Transformation of BBa_B0015(dT), B0034(RBS), I179005(pT7), K346007(Ag43), and K542009(pLacI-RBS-Ag43)in DH5α
- Cultivation on LBA(B0015,RBS) and LBC(Ag43, pLacI-RBS-Ag43)
5th
Transformation
- Transformation of K346007(Ag43)in DH5α
- Cultivation on LBC.
Single colony isolation
- Single colony isolation of parts written above.
- BBa_K542009 was Ag43 only parts! And the part didn't have Biobrick suffix.
6th
Liquid culture
- Liquid culture in LBA(B0015,RBS) and LBC(Ag43)
- LB 3ul: 1ul is for glycerol stocks and 2ul is for mini-prep
