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From 2012.igem.org

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-	<FONT COLOR=#ff6700><b>BioBricks</b></FONT><br><br>
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-	We did not find  urgent  safety  issues with our biobricks. The promoters were taken from the genome of B.subtilis 168 and are natural gene parts. Furthermore the other parts of our biobricks are parts taken from the biobricks in the registry. Also final product does not contain an antibiotic resistance marker.
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Revision as of 14:47, 4 September 2012

During our project discussions and our presentations to the public, it became clear that the association of meat and bacteria being close to each other, is not easily accepted. That’s why decided to take extra care when we designed our indication sticker, to be absolutely sure there is no possibility that our Bacillus or its spores are able to come in the environment or on the preserved meat, possibly endangering the public and environment.

We consulted specialized companies to participate and contribute in our project using their knowledge of materials. A good idea for preventing introduction of GMO’s into the environment, is the use of nano pores. The material for this “sticker” is TPX® Polymethylpentene, a strong polymer that is will ensure no bacteria will not come in contact with the meat, but still allows the volatiles to reach the bacteria. This material is approved by FDA-standards and EU-food-standards.

But not only the sticker design ensures the safety of the public, also the nutrient composition in the sticker ensures Bacillus will only germinate and grow when the sticker is still intact. Even if our Bacillus is exposed to an environment that is favorable for growth the genetic modified insert is not harmful for human, animal or environment. Further the strain we will use in our final product is considered a food-grade strain. More on the safety aspect of the GMO is found in the environmental safety page.
With proper use of the food warden system none of the B. subtilis cells are released. So there is no danger for the public.

The find out more on the effect of our product on the public we want to contact the voedsel en waren authoriteit of the Dutch government.