Team:Groningen/parts fail

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Failed Biobricks


List of failed BioBricks

BBa_K090403

Name: Gram-positive Shuttle Vector for Chromosomal Integration
Intended Purpose: Plasmid backbone for cloning in Bacillus subtilis
Testing Protocol: Restriction analysis (dual cut with EcoRI and PstI)
Results: The restriction pattern showed that this biobrick is not as described in the parts registry. The cut plasmid is not a single band and shorter than the expected size.
Gel Pic



BBa_I742123

Name: Multi-host vector pTG262 converted to BioBrick vector
Intended Purpose: Plasmid backbone for cloning in Bacillus subtilis
Testing Protocol: Restriction analysis (single cut) and restriction analysis (dual cut)
Results: The restriction enzymes cut at illegal site(s)



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