Team:Groningen/parts fail

From 2012.igem.org

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<z1>Failed BioBricks</z1><br>
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<b><font color=#FF6700 size=5pt>List of failed BioBricks</font></b><br>
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<b>BBa_K090403</b><br>
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<b>Name</b> Gram-positive Shuttle Vector for Chromosomal Integration<br>
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<b>Intended Purpose</b> Plasmid backbone for cloning in <i>Bacillus subtilis</i><br>
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<b>Testing Protocol</b> Restriction analysis (single cut)<br>
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<b>Results</b> The restriction pattern showed that this biobrick is not as described in the parts registry. The cut plasmid is shorter than the expected size.<br>
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<b>BBa_I742123</b><br>
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<b>Name</b> multi-host vector pTG262 converted to BioBrick vector<br>
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<b>Intended Purpose</b> plasmid backbone for cloning in <i>Bacilus subtilis</i><br>
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<b>Testing Protocol</b> restriction analysis (single cut) and restriction analysis (dual cut)<br>
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<b>Results</b> the restriction enzymes cut at illegal site(s)<br>
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<body>
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<div class="cte">
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<div class="ctd">
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<z1>Failed Biobricks</z1>
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</div>
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</div>
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<br>
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<br>
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<p>
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<z2>List of failed BioBricks</z2>
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<br>
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<br>
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<b>BBa_K090403</b>
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<br>
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<table class="margintable">
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<td class="textcell90">
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Name:
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</td>
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<td class="textcell90">
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Gram-positive Shuttle Vector for Chromosomal Integration
 +
</td>
 +
</tr>
 +
<tr>
 +
<td class="textcell90">
 +
Intended Purpose:
 +
</td>
 +
<td class="textcell90">
 +
Plasmid backbone for cloning in <i>Bacillus subtilis</i>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td class="textcell90">
 +
Testing Protocol:
 +
</td>
 +
<td class="textcell90">
 +
Restriction analysis (single cut)
 +
</td>
 +
</tr>
 +
<tr>
 +
<td class="textcell90">
 +
Results:
 +
</td>
 +
<td class="textcell90">
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The restriction pattern showed that this biobrick is not as described in the parts registry. The cut plasmid is shorter than the expected size.
 +
</td>
 +
</tr>
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</table>
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<p>
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<br>
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<br>
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<b>BBa_I742123</b>
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<br>
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</p>
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<table class="margintable">
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<tr>
 +
<td class="textcell90">
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Name:
 +
</td>
 +
<td class="textcell90">
 +
Multi-host vector pTG262 converted to BioBrick vector
 +
</td>
 +
</tr>
 +
<tr>
 +
<td class="textcell90">
 +
Intended Purpose:
 +
</td>
 +
<td class="textcell90">
 +
Plasmid backbone for cloning in <i>Bacillus subtilis</i>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td class="textcell90">
 +
Testing Protocol:
 +
</td>
 +
<td class="textcell90">
 +
Restriction analysis (single cut) and restriction analysis (dual cut)
 +
</td>
 +
</tr>
 +
<tr>
 +
<td class="textcell90">
 +
Results:
 +
</td>
 +
<td class="textcell90">
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The restriction enzymes cut at illegal site(s)
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</td>
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</tr>
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</table>
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<br>
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<br>
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<br>
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{{Template:SponsorsGroningen2012}}
{{Template:SponsorsGroningen2012}}

Revision as of 21:10, 25 September 2012





Failed Biobricks


List of failed BioBricks

BBa_K090403

Name: Gram-positive Shuttle Vector for Chromosomal Integration
Intended Purpose: Plasmid backbone for cloning in Bacillus subtilis
Testing Protocol: Restriction analysis (single cut)
Results: The restriction pattern showed that this biobrick is not as described in the parts registry. The cut plasmid is shorter than the expected size.



BBa_I742123

Name: Multi-host vector pTG262 converted to BioBrick vector
Intended Purpose: Plasmid backbone for cloning in Bacillus subtilis
Testing Protocol: Restriction analysis (single cut) and restriction analysis (dual cut)
Results: The restriction enzymes cut at illegal site(s)



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