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From 2012.igem.org

Nisa

Multihost vector (BBa_I742123) double digestion with EcoRI-SpeI and XbaI-PstI.

Result: Gel image of this vector is inconsistent. BBa_I742123 was isolated from 3 different E. coli colonies and was double-digested. The result of this reaction, however, varies between isolates (gel picture will be updated soon).

PCR of alsT promoter region using -150, -250, -300, -500 forward primer.

Last week the annealing temperature of each primer sets has been optimized. We experienced the best result in 58-60oC. Today the PCR was done with different polymerase, Pfu, instead of Taq. Annealing temperature used in this PCR was 60oC.

Result: Only primer pair (Fwd-150,Rev) resulted in DNA fragment in the 0.8% agarose gel. The other primer pairs failed to give the expected results.

Biobrick parts that was requested from iGEM HQ finally arrived!!

List of biobrick parts: 1. BBa_K116603 2. BBa_K116602 3. BBa_J37019 4. BBa_K116639 5. BBa_K116609

E. coli containing these parts were inoculated into LB agar+100ug/ml Ampicilin. Incubate overnight in 37oC