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Team:Groningen/Notebook/Wetwork 8September2012
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| - | + | Nisa <br> <br> | |
| + | Check the plasmid from miniprep yesterday. Cut with 2 restriction enzymes (EcoRI and PstI). <br> | ||
| + | Result: <br> | ||
| + | 1. BBa_K823023+amilGFP OK! <br> | ||
| + | 2. pSac-Cm+amilGFP OK! <br> | ||
| + | 3. pSac-Cm+amilCP OK! <br> | ||
| + | 4. BBa_K823023+lycopene OK! <br> | ||
| + | 5. BBa_K823023+amilCP OK! <br> <br> | ||
| + | Ligated reporters (alsT, fnr, sboA) into backbone + reporter (overnight reaction) | ||
<br> | <br> | ||
<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | <A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | ||
Latest revision as of 14:50, 26 September 2012
Nisa
Check the plasmid from miniprep yesterday. Cut with 2 restriction enzymes (EcoRI and PstI).
Result:
1. BBa_K823023+amilGFP OK!
2. pSac-Cm+amilGFP OK!
3. pSac-Cm+amilCP OK!
4. BBa_K823023+lycopene OK!
5. BBa_K823023+amilCP OK!
Ligated reporters (alsT, fnr, sboA) into backbone + reporter (overnight reaction)
Back to notebook
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