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Team:Groningen/Notebook/Wetwork 8August2012
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(Created page with "{{HeaderGroningen2012}} Elbrich Phusion PCR reaction with violacein DNA, BBa_K274002.") |
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| - | Phusion PCR reaction with violacein DNA, BBa_K274002. | + | Phusion PCR reaction with violacein DNA, BBa_K274002.<br> |
| + | <br> | ||
| + | Nisa <br> | ||
| + | <br> | ||
| + | Ligated fnr and lacI promoter with pigment(s) coding region and introduced the ligation product into E. coli DH5a<br> | ||
| + | List of transformation: <br> | ||
| + | - lacI promoter + amilCP (blue) <br> | ||
| + | - lacI promoter + amilGFP (yellow) <br> | ||
| + | - lacI promoter + lycopene (red) <br> | ||
| + | - fnr promoter + amilCP <br> | ||
| + | - fnr promoter + amilGFP <br> | ||
| + | - fnr promoter + lycopene <br> | ||
| + | <br> | ||
| + | *) lacI promoter was induced by 0.2 and 0.5 mM IPTG | ||
Revision as of 13:45, 9 August 2012
Elbrich
Phusion PCR reaction with violacein DNA, BBa_K274002.
Nisa
Ligated fnr and lacI promoter with pigment(s) coding region and introduced the ligation product into E. coli DH5a
List of transformation:
- lacI promoter + amilCP (blue)
- lacI promoter + amilGFP (yellow)
- lacI promoter + lycopene (red)
- fnr promoter + amilCP
- fnr promoter + amilGFP
- fnr promoter + lycopene
- ) lacI promoter was induced by 0.2 and 0.5 mM IPTG
