Team:Groningen/Notebook/Wetwork 18September2012

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The pcr products of amilcp + fnr, amilcp + sboA and amilgfp + sboA were succesfull. The constructs with lycopene need a longer elongation time. The primers for alsT are probably causing flaws.<br>
The pcr products of amilcp + fnr, amilcp + sboA and amilgfp + sboA were succesfull. The constructs with lycopene need a longer elongation time. The primers for alsT are probably causing flaws.<br>
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Arjan
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miniprep of psbic3 with alst+amilCP and  SboA+cmilCP/amilGFP and lycopene. Restriction control with EcoRI and PstI. All the construct with SboA are good and will be sent tomorrow.
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  <A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
  <A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
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Revision as of 15:14, 26 September 2012







Tom

Colony pcr with taq polymerase for the constructs: amilcp + alsT, lycopene + alsT, amilcp + fnr, lycopene + fnr, amilcp + sboA, lycopene + sboA and amilgfp + sboA.

The pcr products of amilcp + fnr, amilcp + sboA and amilgfp + sboA were succesfull. The constructs with lycopene need a longer elongation time. The primers for alsT are probably causing flaws.

Arjan
miniprep of psbic3 with alst+amilCP and SboA+cmilCP/amilGFP and lycopene. Restriction control with EcoRI and PstI. All the construct with SboA are good and will be sent tomorrow.

Back to notebook