Team:Groningen/DesignTest2

From 2012.igem.org

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{{HeaderGroningen2012}}  
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{{HeaderGroningen2012}}
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</style>
</head>
</head>
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</html>
 
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<html>
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<body>
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<div class="cte">
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<div class="cte">
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<div class="ctd">
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<div class="ctd">
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<z1>Wetwork</z1><br>
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<z1>Abstract</z1>
 +
</div>
</div>
</div>
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</div>
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<table class="centertable">
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<table class="calendargroup">
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<tr>
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<tr>
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<td class="margincell" align="right">
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<td>
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<div class="bigcog" id="bigcogtopleft">
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<table class="calendarheader">
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<img src="http://2012.igem.org/wiki/images/b/b6/Groningen2012_AD_20120802_BigCog.png" width="150px" height="150px">
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<tr class="monthheader">
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</div>
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<td colspan=7 >
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<div class="cogoverlay" id="cogoverlaytopleft">
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<div class="mte">
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<img src="http://2012.igem.org/wiki/images/1/15/Groningen2012_RR20120909_construct.png" width="100px" height="100px">
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<div class="mtd">
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</div>
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<mh>April</mh><br>
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</td>
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</div>
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<td colspan="2">
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</div>
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<p class="nomargin">
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</td>
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Every year, one third of global food production -1.3 billion tons of food- is thrown away, partially due to the “best before” dating system.
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</tr>
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<z7>iGEM Groningen 2012</z7> seeks to provide an alternative method of assessing edibility: the <z7>Food Warden</z7>. It uses an <z7>engineered strain</z7>  
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<tr class="dayheader">
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of <i>Bacillus subtilis</i> to detect and report volatiles in spoiling meat. The introduced <z7>genetic construct</z7> uses a promoter to trigger
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                        <td class="daylabelend">Su</td>
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a pigment coding gene. This promoter, <z7>identified by microarray analysis</z7>, is significantly upregulated in the presence of
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                        <td class="daylabel">M</td>
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<z7>volatiles from spoiling meat</z7>. The activity of the <z7>promoter</z7> regulates the expression of the <z7>pigment reporter</z7> and will
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                        <td class="daylabel">Tu</td>
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be visible to the naked eye. For safe usage of the system, spores of our engineered strain are placed into one half of a semi-permeable
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                        <td class="daylabel">W</td>
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<z7>capsule</z7>, the second containing a calibrated amount of nutrients. Breaking the barrier between the two compartments allows
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                        <td class="daylabel">Th</td>
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<z7>germination and growth</z7>, thereby activating the <z7>spoiling-meat sensor</z7>.
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                        <td class="daylabel">F</td>
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                        <td class="daylabelend">Sa</td>
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                    </tr>
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</table>
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<table class="calendar">
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<tr>
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<td class="day">1</td>
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<td class="day">2</td>
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<td class="day">3</td>
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<td class="day">4</td>
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<td class="day">5</td>
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<td class="day">6</td>
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<td class="day">7</td>
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</tr>
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<tr>
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<td class="day">8</td>
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<td class="day">9</td>
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<td class="day">10</td>
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<td class="day">11</td>
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<td class="day">12</td>
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<td class="day">13</td>
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<td class="day">14</td>
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</tr>
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<tr>
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<td class="day">15</td>
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<td class="day">16</td>
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<td class="day">17</td>
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<td class="day">18</td>
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<td class="day">19</td>
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<td class="day">20</td>
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<td class="day">21</td>
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</tr>
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<tr>
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<td class="day">22</td>
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<td class="day">23</td>
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<td class="day">24</td>
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<td class="day">25</td>
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<td class="day">26</td>
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<td class="day">27</td>
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<td class="day">28</td>
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</tr>
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<tr>
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<td class="day">29</td>
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<td class="day">30</td>
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<td class="day"> </td>
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<td class="day"> </td>
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<td class="day"> </td>
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<td class="day"> </td>
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</tr>
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</table>
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</td>
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<td>
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<table class="calendarheader">
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<tr class="monthheader">
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<td colspan=7 >
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<div class="mte">
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<div class="mtd">
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<mh>May</mh><br>
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</div>
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</div>
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</td>
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</tr>
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<tr class="dayheader">
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                        <td class="daylabelend">Su</td>
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                        <td class="daylabel">M</td>
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                        <td class="daylabel">Tu</td>
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                        <td class="daylabel">W</td>
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                        <td class="daylabel">Th</td>
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                        <td class="daylabel">F</td>
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                        <td class="daylabelend">Sa</td>
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                    </tr>
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</table>
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<table class="calendar">
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<tr>
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<td class="day"> </td>
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<td class="day"> </td>
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<td class="day">1</td>
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<td class="day">2</td>
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<td class="day">3</td>
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<td class="day">4</td>
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<td class="day">5</td>
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</tr>
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<tr>
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<td class="day">6</td>
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<td class="day">7</td>
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<td class="day">8</td>
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<td class="day">9</td>
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<td class="day">10</td>
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<td class="day">11</td>
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<td class="day">12</td>
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</tr>
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<tr>
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<td class="day">13</td>
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<td class="day">14</td>
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<td class="day">15</td>
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<td class="day">16</td>
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<td class="day">17</td>
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<td class="day">18</td>
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<td class="day">19</td>
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</tr>
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<tr>
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<td class="day">20</td>
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<td class="day">21</td>
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<td class="day">22</td>
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<td class="day">23</td>
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<td class="day">24</td>
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<td class="day">25</td>
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<td class="day">26</td>
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</tr>
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<tr>
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<td class="day">27</td>
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<td class="day">28</td>
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<td class="day">29</td>
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<td class="day">30</td>
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<td class="day">31</td>
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<td class="day"> </td>
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<td class="day"> </td>
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</tr>
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</table>
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</td>
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<td>
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<table class="calendarheader">
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<tr class="monthheader">
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<td colspan=7 >
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<div class="mte">
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<div class="mtd">
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<mh>June</mh><br>
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</div>
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</div>
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</td>
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</tr>
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<tr class="dayheader">
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                        <td class="daylabelend">Su</td>
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                        <td class="daylabel">M</td>
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                        <td class="daylabel">Tu</td>
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                        <td class="daylabel">W</td>
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                        <td class="daylabel">Th</td>
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                        <td class="daylabel">F</td>
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                        <td class="daylabelend">Sa</td>
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                    </tr>
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</table>
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<table class="calendar">
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<tr>
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<td class="day"> </td>
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<td class="day"> </td>
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<td class="day"> </td>
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<td class="day"> </td>
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<td class="day"> </td>
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<td class="day">1</td>
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<td class="day">2</td>
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</tr>
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<tr>
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<td class="day">3</td>
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<td class="day">4</td>
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<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_5June2012">5</a></td>
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<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_6June2012">6</a></td>
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<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_7June2012">7</a></td>
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<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_8June2012">8</a></td>
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<td class="day">9</td>
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</tr>
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<tr>
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<td class="day">10</td>
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<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_11June2012">11</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_12June2012">12</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_13June2012">13</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_14June2012">14</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_15June2012">15</a></td>
+
-
<td class="day">16</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">17</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_18June2012">18</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_19June2012">19</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_20June2012">20</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_21June2012">21</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_22June2012">22</a></td>
+
-
<td class="day">23</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">24</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_25June2012">25</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_26June2012">26</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_27June2012">27</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_28June2012">28</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_29June2012">29</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_30June2012">30</a></td>
+
-
</tr>
+
-
</table>
+
-
</td>
+
-
<tr>
+
-
</tr>
+
-
<td>
+
-
<table class="calendarheader">
+
-
<tr class="monthheader">
+
-
<td colspan=7 >
+
-
<div class="mte">
+
-
<div class="mtd">
+
-
<mh>July</mh><br>
+
-
</div>
+
-
</div>
+
-
</td>
+
-
</tr>
+
-
<tr class="dayheader">
+
-
                        <td class="daylabelend">Su</td>
+
-
                        <td class="daylabel">M</td>
+
-
                        <td class="daylabel">Tu</td>
+
-
                        <td class="daylabel">W</td>
+
-
                        <td class="daylabel">Th</td>
+
-
                        <td class="daylabel">F</td>
+
-
                        <td class="daylabelend">Sa</td>
+
-
                    </tr>
+
-
</table>
+
-
<table class="calendar">
+
-
<tr>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_1July2012">1</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_2July2012">2</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_3July2012">3</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_4July2012">4</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_5July2012">5</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_6July2012">6</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_7July2012">7</a></td>
+
-
</tr>
+
-
<tr>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_8July2012">8</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_9July2012">9</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_10July2012">10</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_11July2012">11</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_12July2012">12</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_13July2012">13</a></td>
+
-
<td class="day">14</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">15</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_16July2012">16</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_17July2012">17</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_18July2012">18</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_19July2012">19</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_20July2012">20</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_21July2012">21</a></td>
+
-
</tr>
+
-
<tr>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_22July2012">22</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_23July2012">23</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_24July2012">24</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_25July2012">25</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_26July2012">26</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_27July2012">27</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_28July2012">28</a></td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">29</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_30July2012">30</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_31July2012">31</a></td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
</tr>
+
-
</table>
+
-
</td>
+
-
<td>
+
-
<table class="calendarheader">
+
-
<tr class="monthheader">
+
-
<td colspan=7 >
+
-
<div class="mte">
+
-
<div class="mtd">
+
-
<mh>August</mh><br>
+
-
</div>
+
-
</div>
+
-
</td>
+
-
</tr>
+
-
<tr class="dayheader">
+
-
                        <td class="daylabelend">Su</td>
+
-
                        <td class="daylabel">M</td>
+
-
                        <td class="daylabel">Tu</td>
+
-
                        <td class="daylabel">W</td>
+
-
                        <td class="daylabel">Th</td>
+
-
                        <td class="daylabel">F</td>
+
-
                        <td class="daylabelend">Sa</td>
+
-
                    </tr>
+
-
</table>
+
-
<table class="calendar">
+
-
<tr>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_1August2012">1</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_2August2012">2</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_3August2012">3</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_4August2012">4</a></td>
+
-
</tr>
+
-
<tr>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_5August2012">5</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_6August2012">6</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_7August2012">7</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_8August2012">8</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_9August2012">9</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_10August2012">10</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_11August2012">11</a></td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">12</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_13August2012">13</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_14August2012">14</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_15August2012">15</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_16August2012">16</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_17August2012">17</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_18August2012">18</a></td>
+
-
</tr>
+
-
<tr>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_19August2012">19</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_20August2012">20</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_21August2012">21</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_22August2012">22</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_23August2012">23</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_24August2012">24</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_25August2012">25</a></td>
+
-
</tr>
+
-
<tr>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_26August2012">26</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_27August2012">27</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_28August2012">28</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_29August2012">29</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_30August2012">30</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_31August2012">31</a></td>
+
-
<td class="day"> </td>
+
-
</tr>
+
-
</table>
+
-
</td>
+
-
<td>
+
-
<table class="calendarheader">
+
-
<tr class="monthheader">
+
-
<td colspan=7 >
+
-
<div class="mte">
+
-
<div class="mtd">
+
-
<mh>September</mh><br>
+
-
</div>
+
-
</div>
+
-
</td>
+
-
</tr>
+
-
<tr class="dayheader">
+
-
                        <td class="daylabelend">Su</td>
+
-
                        <td class="daylabel">M</td>
+
-
                        <td class="daylabel">Tu</td>
+
-
                        <td class="daylabel">W</td>
+
-
                        <td class="daylabel">Th</td>
+
-
                        <td class="daylabel">F</td>
+
-
                        <td class="daylabelend">Sa</td>
+
-
                    </tr>
+
-
</table>
+
-
<table class="calendar">
+
-
<tr>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_1September2012">1</a></td>
+
-
</tr>
+
-
<tr>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_2September2012">2</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_3September2012">3</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_4September2012">4</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_5September2012">5</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_6September2012">6</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_7September2012">7</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_8September2012">8</a></td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">9</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_10September2012">10</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_11September2012">11</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_12September2012">12</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_13September2012">13</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_14September2012">14</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_15September2012">15</a></td>
+
-
</tr>
+
-
<tr>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_16September2012">16</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_17September2012">17</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_18September2012">18</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_19September2012">19</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_20September2012">20</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_21September2012">21</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_22September2012">22</a></td>
+
-
</tr>
+
-
<tr>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_23September2012">23</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_24September2012">24</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_25September2012">25</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Wetwork_26September2012">26</a></td>
+
-
<td class="day">27</td>
+
-
<td class="day">28</td>
+
-
<td class="day">29</td>
+
-
</tr>
+
-
</table>
+
-
</td>
+
-
</tr>
+
-
</table>
+
-
<p class="z5">
+
-
The protocols we used daily, can be found <a class="inlink" href="http://2012.igem.org/Team:Groningen/protocol">here</a>.
+
-
</p>
+
-
<div class="cte">
+
-
<div class="ctd">
+
-
<z1>Modeling</z1><br>
+
-
</div>
+
-
</div>
+
-
<table class="calendargroup">
+
-
<tr>
+
-
<td>
+
-
<table class="calendarheader">
+
-
<tr class="monthheader">
+
-
<td colspan=7 >
+
-
<div class="mte">
+
-
<div class="mtd">
+
-
<mh>March</mh><br>
+
-
</div>
+
-
</div>
+
-
</td>
+
-
</tr>
+
-
<tr class="dayheader">
+
-
                        <td class="daylabelend">Su</td>
+
-
                        <td class="daylabel">M</td>
+
-
                        <td class="daylabel">Tu</td>
+
-
                        <td class="daylabel">W</td>
+
-
                        <td class="daylabel">Th</td>
+
-
                        <td class="daylabel">F</td>
+
-
                        <td class="daylabelend">Sa</td>
+
-
                    </tr>
+
-
</table>
+
-
<table class="calendar">
+
-
<tr>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day">1</td>
+
-
<td class="day">2</td>
+
-
<td class="day">3</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">4</td>
+
-
<td class="day">5</td>
+
-
<td class="day">6</td>
+
-
<td class="day">7</td>
+
-
<td class="day">8</td>
+
-
<td class="day">9</td>
+
-
<td class="day">10</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">11</td>
+
-
<td class="day">12</td>
+
-
<td class="day">13</td>
+
-
<td class="day">14</td>
+
-
<td class="day">15</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Modeling_16March2012">16</a></td>
+
-
<td class="day">17</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">18</td>
+
-
<td class="day">19</td>
+
-
<td class="day">20</td>
+
-
<td class="day">21</td>
+
-
<td class="day">22</td>
+
-
<td class="day">23</td>
+
-
<td class="day">24</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">25</td>
+
-
<td class="day">26</td>
+
-
<td class="day">27</td>
+
-
<td class="day">28</td>
+
-
<td class="day">29</td>
+
-
<td class="day">30</td>
+
-
<td class="day">31</td>
+
-
</tr>
+
-
</table>
+
-
</td>
+
-
<td>
+
-
<table class="calendarheader">
+
-
<tr class="monthheader">
+
-
<td colspan=7 >
+
-
<div class="mte">
+
-
<div class="mtd">
+
-
<mh>April</mh><br>
+
-
</div>
+
-
</div>
+
-
</td>
+
-
</tr>
+
-
<tr class="dayheader">
+
-
                        <td class="daylabelend">Su</td>
+
-
                        <td class="daylabel">M</td>
+
-
                        <td class="daylabel">Tu</td>
+
-
                        <td class="daylabel">W</td>
+
-
                        <td class="daylabel">Th</td>
+
-
                        <td class="daylabel">F</td>
+
-
                        <td class="daylabelend">Sa</td>
+
-
                    </tr>
+
-
</table>
+
-
<table class="calendar">
+
-
<tr>
+
-
<td class="day">1</td>
+
-
<td class="day">2</td>
+
-
<td class="day">3</td>
+
-
<td class="day">4</td>
+
-
<td class="day">5</td>
+
-
<td class="day">6</td>
+
-
<td class="day">7</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">8</td>
+
-
<td class="day">9</td>
+
-
<td class="day">10</td>
+
-
<td class="day">11</td>
+
-
<td class="day">12</td>
+
-
<td class="day">13</td>
+
-
<td class="day">14</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">15</td>
+
-
<td class="day">16</td>
+
-
<td class="day">17</td>
+
-
<td class="day">18</td>
+
-
<td class="day">19</td>
+
-
<td class="day">20</td>
+
-
<td class="day">21</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">22</td>
+
-
<td class="day">23</td>
+
-
<td class="day">24</td>
+
-
<td class="day">25</td>
+
-
<td class="day">26</td>
+
-
<td class="day">27</td>
+
-
<td class="day">28</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">29</td>
+
-
<td class="day">30</td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
</tr>
+
-
</table>
+
-
</td>
+
-
<td>
+
-
<table class="calendarheader">
+
-
<tr class="monthheader">
+
-
<td colspan=7 >
+
-
<div class="mte">
+
-
<div class="mtd">
+
-
<mh>May</mh><br>
+
-
</div>
+
-
</div>
+
-
</td>
+
-
</tr>
+
-
<tr class="dayheader">
+
-
                        <td class="daylabelend">Su</td>
+
-
                        <td class="daylabel">M</td>
+
-
                        <td class="daylabel">Tu</td>
+
-
                        <td class="daylabel">W</td>
+
-
                        <td class="daylabel">Th</td>
+
-
                        <td class="daylabel">F</td>
+
-
                        <td class="daylabelend">Sa</td>
+
-
                    </tr>
+
-
</table>
+
-
<table class="calendar">
+
-
<tr>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day">1</td>
+
-
<td class="day">2</td>
+
-
<td class="day">3</td>
+
-
<td class="day">4</td>
+
-
<td class="day">5</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">6</td>
+
-
<td class="day">7</td>
+
-
<td class="day">8</td>
+
-
<td class="day">9</td>
+
-
<td class="day">10</td>
+
-
<td class="day">11</td>
+
-
<td class="day">12</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">13</td>
+
-
<td class="day">14</td>
+
-
<td class="day">15</td>
+
-
<td class="day">16</td>
+
-
<td class="day">17</td>
+
-
<td class="day">18</td>
+
-
<td class="day">19</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">20</td>
+
-
<td class="day">21</td>
+
-
<td class="day">22</td>
+
-
<td class="day">23</td>
+
-
<td class="day">24</td>
+
-
<td class="day">25</td>
+
-
<td class="day">26</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">27</td>
+
-
<td class="day">28</td>
+
-
<td class="day">29</td>
+
-
<td class="day">30</td>
+
-
<td class="day">31</td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
</tr>
+
-
</table>
+
-
</td>
+
-
<td>
+
-
<table class="calendarheader">
+
-
<tr class="monthheader">
+
-
<td colspan=7 >
+
-
<div class="mte">
+
-
<div class="mtd">
+
-
<mh>June</mh><br>
+
-
</div>
+
-
</div>
+
-
</td>
+
-
</tr>
+
-
<tr class="dayheader">
+
-
                        <td class="daylabelend">Su</td>
+
-
                        <td class="daylabel">M</td>
+
-
                        <td class="daylabel">Tu</td>
+
-
                        <td class="daylabel">W</td>
+
-
                        <td class="daylabel">Th</td>
+
-
                        <td class="daylabel">F</td>
+
-
                        <td class="daylabelend">Sa</td>
+
-
                    </tr>
+
-
</table>
+
-
<table class="calendar">
+
-
<tr>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day">1</td>
+
-
<td class="day">2</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">3</td>
+
-
<td class="day">4</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Modeling_5June2012">5</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Modeling_6June2012">6</a></td>
+
-
<td class="day">7</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Modeling_8June2012">8</a></td>
+
-
<td class="day">9</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">10</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Modeling_11June2012">11</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Modeling_12June2012">12</a></td>
+
-
<td class="day">13</td>
+
-
<td class="day">14</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Modeling_15June2012">15</a></td>
+
-
<td class="day">16</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">17</td>
+
-
<td class="day">18</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Modeling_19June2012">19</a></td>
+
-
<td class="day">20</td>
+
-
<td class="day">21</td>
+
-
<td class="day">22</td>
+
-
<td class="day">23</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">24</td>
+
-
<td class="day">25</td>
+
-
<td class="day">26</td>
+
-
<td class="day">27</td>
+
-
<td class="day">28</td>
+
-
<td class="day">29</td>
+
-
<td class="day">30</td>
+
-
</tr>
+
-
</table>
+
-
</td>
+
-
<tr>
+
-
</tr>
+
-
<td colspan=4>
+
-
<table style="margin: 0 auto; background-color: transparent;">
+
-
<tr>
+
-
<td>
+
-
<table class="calendarheader">
+
-
<tr class="monthheader">
+
-
<td colspan=7 >
+
-
<div class="mte">
+
-
<div class="mtd">
+
-
<mh>July</mh><br>
+
-
</div>
+
-
</div>
+
-
</td>
+
-
</tr>
+
-
<tr class="dayheader">
+
-
<td class="daylabelend">Su</td>
+
-
<td class="daylabel">M</td>
+
-
<td class="daylabel">Tu</td>
+
-
<td class="daylabel">W</td>
+
-
<td class="daylabel">Th</td>
+
-
<td class="daylabel">F</td>
+
-
<td class="daylabelend">Sa</td>
+
-
</tr>
+
-
</table>
+
-
<table class="calendar">
+
-
<tr>
+
-
<td class="day">1</td>
+
-
<td class="day">2</td>
+
-
<td class="day">3</td>
+
-
<td class="day">4</td>
+
-
<td class="day">5</td>
+
-
<td class="day">6</td>
+
-
<td class="day">7</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">8</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Modeling_9July2012">9</a></td>
+
-
<td class="day">10</td>
+
-
<td class="day">11</td>
+
-
<td class="day">12</td>
+
-
<td class="day">13</td>
+
-
<td class="day">14</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">15</td>
+
-
<td class="day">16</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Modeling_17July2012">17</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Modeling_18July2012">18</a></td>
+
-
<td class="day">19</td>
+
-
<td class="day">20</td>
+
-
<td class="day">21</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">22</td>
+
-
<td class="day">23</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Modeling_24July2012">24</a></td>
+
-
<td class="day">25</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Modeling_26July2012">26</a></td>
+
-
<td class="day">27</td>
+
-
<td class="day">28</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">29</td>
+
-
<td class="day">30</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Modeling_31July2012">31</a></td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
</tr>
+
-
</table>
+
-
</td>
+
-
<td>
+
-
<table class="calendarheader">
+
-
<tr class="monthheader">
+
-
<td colspan=7 >
+
-
<div class="mte">
+
-
<div class="mtd">
+
-
<mh>August</mh><br>
+
-
</div>
+
-
</div>
+
-
</td>
+
-
</tr>
+
-
<tr class="dayheader">
+
-
<td class="daylabelend">Su</td>
+
-
<td class="daylabel">M</td>
+
-
<td class="daylabel">Tu</td>
+
-
<td class="daylabel">W</td>
+
-
<td class="daylabel">Th</td>
+
-
<td class="daylabel">F</td>
+
-
<td class="daylabelend">Sa</td>
+
-
</tr>
+
-
</table>
+
-
<table class="calendar">
+
-
<tr>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day">1</td>
+
-
<td class="day">2</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Modeling_3August2012">3</a></td>
+
-
<td class="day">4</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">5</td>
+
-
<td class="day">6</td>
+
-
<td class="day">7</td>
+
-
<td class="day">8</td>
+
-
<td class="day">9</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Modeling_10August2012">10</a></td>
+
-
<td class="day">11</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Modeling_12August2012">12</a></td>
+
-
<td class="day">13</td>
+
-
<td class="day">14</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Modeling_15August2012">15</a></td>
+
-
<td class="day">16</td>
+
-
<td class="day">17</td>
+
-
<td class="day">18</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">19</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Modeling_20August2012">20</a></td>
+
-
<td class="day">21</td>
+
-
<td class="day">22</td>
+
-
<td class="day">23</td>
+
-
<td class="day">24</td>
+
-
<td class="day">25</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">26</td>
+
-
<td class="day">27</td>
+
-
<td class="day">28</td>
+
-
<td class="day">29</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Modeling_30August2012">30</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Modeling_31August2012">31</a></td>
+
-
<td class="day"> </td>
+
-
</tr>
+
-
</table>
+
-
</td>
+
-
<td>
+
-
<table class="calendarheader">
+
-
<tr class="monthheader">
+
-
<td colspan=7 >
+
-
<div class="mte">
+
-
<div class="mtd">
+
-
<mh>September</mh><br>
+
-
</div>
+
-
</div>
+
-
</td>
+
-
</tr>
+
-
<tr class="dayheader">
+
-
<td class="daylabelend">Su</td>
+
-
<td class="daylabel">M</td>
+
-
<td class="daylabel">Tu</td>
+
-
<td class="daylabel">W</td>
+
-
<td class="daylabel">Th</td>
+
-
<td class="daylabel">F</td>
+
-
<td class="daylabelend">Sa</td>
+
-
</tr>
+
-
</table>
+
-
<table class="calendar">
+
-
<tr>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day">1</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">2</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Modeling_3September2012">3</a></td>
+
-
<td class="day">4</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Modeling_5September2012">5</a></td>
+
-
<td class="day">6</td>
+
-
<td class="day">7</td>
+
-
<td class="day">8</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">9</td>
+
-
<td class="day">10</td>
+
-
<td class="day">11</td>
+
-
<td class="day">12</td>
+
-
<td class="day">13</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/Modeling_14September2012">14</a></td>
+
-
<td class="day">15</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">16</td>
+
-
<td class="day">17</td>
+
-
<td class="day">18</td>
+
-
<td class="day">19</td>
+
-
<td class="day">20</td>
+
-
<td class="day">21</td>
+
-
<td class="day">22</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">23</td>
+
-
<td class="day">24</td>
+
-
<td class="day">25</td>
+
-
<td class="day">26</td>
+
-
<td class="day">27</td>
+
-
<td class="day">28</td>
+
-
<td class="day">29</td>
+
-
</tr>
+
-
</table>
+
-
</td>
+
-
</tr>
+
-
</table>
+
-
</td>
+
-
</tr>
+
-
</table>
+
-
<div class="cte">
+
</p>
-
<div class="ctd">
+
</td>
-
<z1>Human Practice</z1><br>
+
<td class="margincell" align="left">
 +
<div class="bigcog" id="bigcogtopright">
 +
<img src="http://2012.igem.org/wiki/images/b/b6/Groningen2012_AD_20120802_BigCog.png" width="150px" height="150px">
 +
</div>
 +
<div class="cogoverlay" id="cogoverlaytopright">
 +
<img src="http://2012.igem.org/wiki/images/2/29/Groningen2012_RR20120909_badmeat.png" width="100px" height="100px">
 +
</div>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td class="margincell">
 +
<div class="cogoverlay" id="cogoverlaybottomleft">
 +
<img src="http://2012.igem.org/wiki/images/c/c2/Groningen2012_RR_20120909_rotting.png" width="100" height="100">
 +
</div>
 +
</td>
 +
<td align="left" >
 +
<div class="bigcog" id="bigcogbottomleft">
 +
<img src="http://2012.igem.org/wiki/images/b/b6/Groningen2012_AD_20120802_BigCog.png" width="150px" height="150px">
 +
</div>
 +
</td>
 +
<td align="right">
 +
<div class="bigcog" id="bigcogbottomright">
 +
<img src="http://2012.igem.org/wiki/images/b/b6/Groningen2012_AD_20120802_BigCog.png" width="150px" height="150px">
 +
</div>
 +
</td>
 +
<td class="margincell">
 +
<div class="cogoverlay" id="cogoverlaybottomright">
 +
<img src="http://2012.igem.org/wiki/images/e/e7/Groningen2012_RR20120909_capsule.png" width="100px" height="100px">
 +
</div>
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<br>
 +
<div class="cte2">
 +
<div class="ctd2">
 +
<a name="MainAcc"></a><z2 >Our main accomplishments</z2><br>
 +
</div>
</div>
</div>
-
</div>
+
<br><br>
-
<table class="calendargroup">
+
<p class=orange>In the lab</p>
-
<tr>
+
<p class="marginleft">
-
<td>
+
<img src="http://2012.igem.org/wiki/images/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> Most importantly: we developed a construct which makes <i>Bacillus subtilis</i> sense spoiled meat and produce an output in the form of a yellow pigment visible by naked eye.<br><br>
-
<table class="calendarheader">
+
<img src="http://2012.igem.org/wiki/images/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> Design of the “<a href="http://2012.igem.org/Team:Groningen/Sticker">Sticker</a>: semi-permeable capsule: bacteria are kept inside, volatiles can go through. Proof that <i>Bacillus subtilis</i> grows inside the sticker.<br><br>
-
<tr class="monthheader">
+
<img src="http://2012.igem.org/wiki/images/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> Development of BioBrick psac-cm for <i>Bacillus subtilis</i>. <br><a href="http://partsregistry.org/Part:BBa_K818000" target=_blank><font size=4 color=#FF6700><b>Easy cloning in <i>Bacillus subtilis</i> in Biobrick fashion for the first time!</b></font></a> Advantages: easy to check, BioBrick compatible, <i>E. coli</i> compatible, stable insertion into <i>Bacillus subtilis</i> chromosome.<br><br>
-
<td colspan=7 >
+
<img src="http://2012.igem.org/wiki/images/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> Identification of spoiled meat sensors by transcriptome analysis. Usage of an innovative experimental setup which can be used to test more sensors in the future.<br><br>
-
<div class="mte">
+
<img src="http://2012.igem.org/wiki/images/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> We made AmilCP and AmilGFP be expressed in <i>Bacillus subtilis</i>. These chromoproteins can be of significant value to the other <i>Bacillus subtilis</i> users in the BioBrick community.<br><br><br>
-
<div class="mtd">
+
</p>
-
<mh>March</mh><br>
+
<p class=orange>Other</p>
-
</div>
+
<p class="marginleft">
-
</div>
+
<img src="http://2012.igem.org/wiki/images/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> Smart spores activation system: inspired by "glow in the dark stick", we designed a sticker with two compartments. One compartment holds the spores, the other the nutrients; when the nutrients compartment is braked, the spores start to germinate, activating the sticker.<br><br>
-
</td>
+
<img src="http://2012.igem.org/wiki/images/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> We explored the definition of spoiled meat and different ways to test this.<br><br>
-
</tr>
+
<img src="http://2012.igem.org/wiki/images/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> We identified various compounds present in spoiled meat by Gas Chromatography - Mass Spectrometry.<br><br>
-
<tr class="dayheader">
+
<img src="http://2012.igem.org/wiki/images/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> We made a model which gives insight on how to tweak the growth of <i>Bacillus subtilis</i> based upon literature, experimental data and flux balance analysis.<br>
-
                        <td class="daylabelend">Su</td>
+
</p>
-
                        <td class="daylabel">M</td>
+
</body>
-
                        <td class="daylabel">Tu</td>
+
</html>
-
                        <td class="daylabel">W</td>
+
-
                        <td class="daylabel">Th</td>
+
-
                        <td class="daylabel">F</td>
+
-
                        <td class="daylabelend">Sa</td>
+
-
                    </tr>
+
-
</table>
+
-
<table class="calendar">
+
-
<tr>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day">1</td>
+
-
<td class="day">2</td>
+
-
<td class="day">3</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">4</td>
+
-
<td class="day">5</td>
+
-
<td class="day">6</td>
+
-
<td class="day">7</td>
+
-
<td class="day">8</td>
+
-
<td class="day">9</td>
+
-
<td class="day">10</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">11</td>
+
-
<td class="day">12</td>
+
-
<td class="day">13</td>
+
-
<td class="day">14</td>
+
-
<td class="day">15</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_16March2012">16</a></td>
+
-
<td class="day">17</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">18</td>
+
-
<td class="day">19</td>
+
-
<td class="day">20</td>
+
-
 
+
-
<td class="day">21</td>
+
-
<td class="day">22</td>
+
-
<td class="day">23</td>
+
-
<td class="day">24</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">25</td>
+
-
<td class="day">26</td>
+
-
<td class="day">27</td>
+
-
<td class="day">28</td>
+
-
<td class="day">29</td>
+
-
<td class="day">30</td>
+
-
<td class="day">31</td>
+
-
</tr>
+
-
</table>
+
-
</td>
+
-
<td>
+
-
<table class="calendarheader">
+
-
<tr class="monthheader">
+
-
<td colspan=7 >
+
-
<div class="mte">
+
-
<div class="mtd">
+
-
<mh>April</mh><br>
+
-
</div>
+
-
</div>
+
-
</td>
+
-
</tr>
+
-
<tr class="dayheader">
+
-
                        <td class="daylabelend">Su</td>
+
-
                        <td class="daylabel">M</td>
+
-
                        <td class="daylabel">Tu</td>
+
-
                        <td class="daylabel">W</td>
+
-
                        <td class="daylabel">Th</td>
+
-
                        <td class="daylabel">F</td>
+
-
                        <td class="daylabelend">Sa</td>
+
-
                    </tr>
+
-
</table>
+
-
<table class="calendar">
+
-
<tr>
+
-
<td class="day">1</td>
+
-
<td class="day">2</td>
+
-
<td class="day">3</td>
+
-
<td class="day">4</td>
+
-
<td class="day">5</td>
+
-
<td class="day">6</td>
+
-
<td class="day">7</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">8</td>
+
-
<td class="day">9</td>
+
-
<td class="day">10</td>
+
-
<td class="day">11</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_12April2012">12</a></td>
+
-
<td class="day">13</td>
+
-
<td class="day">14</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">15</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_16April2012">16</a></td>
+
-
<td class="day">17</td>
+
-
<td class="day">18</td>
+
-
<td class="day">19</td>
+
-
<td class="day">20</td>
+
-
<td class="day">21</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">22</td>
+
-
<td class="day">23</td>
+
-
<td class="day">24</td>
+
-
<td class="day">25</td>
+
-
<td class="day">26</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_27April2012">27</a></td>
+
-
<td class="day">28</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">29</td>
+
-
<td class="day">30</td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
</tr>
+
-
</table>
+
-
</td>
+
-
<td>
+
-
<table class="calendarheader">
+
-
<tr class="monthheader">
+
-
<td colspan=7 >
+
-
<div class="mte">
+
-
<div class="mtd">
+
-
<mh>May</mh><br>
+
-
</div>
+
-
</div>
+
-
</td>
+
-
</tr>
+
-
<tr class="dayheader">
+
-
                        <td class="daylabelend">Su</td>
+
-
                        <td class="daylabel">M</td>
+
-
                        <td class="daylabel">Tu</td>
+
-
                        <td class="daylabel">W</td>
+
-
                        <td class="daylabel">Th</td>
+
-
                        <td class="daylabel">F</td>
+
-
                        <td class="daylabelend">Sa</td>
+
-
                    </tr>
+
-
</table>
+
-
<table class="calendar">
+
-
<tr>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day">1</td>
+
-
<td class="day">2</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_3May2012">3</a></td>
+
-
<td class="day">4</td>
+
-
<td class="day">5</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">6</td>
+
-
<td class="day">7</td>
+
-
<td class="day">8</td>
+
-
<td class="day">9</td>
+
-
<td class="day">10</td>
+
-
<td class="day">11</td>
+
-
<td class="day">12</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">13</td>
+
-
<td class="day">14</td>
+
-
<td class="day">15</td>
+
-
<td class="day">16</td>
+
-
<td class="day">17</td>
+
-
<td class="day">18</td>
+
-
<td class="day">19</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">20</td>
+
-
<td class="day">21</td>
+
-
<td class="day">22</td>
+
-
<td class="day">23</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_24May2012">24</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_25May2012">25</a></td>
+
-
<td class="day">26</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">27</td>
+
-
<td class="day">28</td>
+
-
<td class="day">29</td>
+
-
<td class="day">30</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_31May2012">31</a></td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
</tr>
+
-
</table>
+
-
</td>
+
-
<td>
+
-
<table class="calendarheader">
+
-
<tr class="monthheader">
+
-
<td colspan=7 >
+
-
<div class="mte">
+
-
<div class="mtd">
+
-
<mh>June</mh><br>
+
-
</div>
+
-
</div>
+
-
</td>
+
-
</tr>
+
-
<tr class="dayheader">
+
-
                        <td class="daylabelend">Su</td>
+
-
                        <td class="daylabel">M</td>
+
-
                        <td class="daylabel">Tu</td>
+
-
                        <td class="daylabel">W</td>
+
-
                        <td class="daylabel">Th</td>
+
-
                        <td class="daylabel">F</td>
+
-
                        <td class="daylabelend">Sa</td>
+
-
                    </tr>
+
-
</table>
+
-
<table class="calendar">
+
-
<tr>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_1June2012">1</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_2June2012">2</a></td>
+
-
</tr>
+
-
<tr>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_3June2012">3</a></td>
+
-
<td class="day">4</td>
+
-
<td class="day">5</td>
+
-
<td class="day">6</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_7June2012">7</a></td>
+
-
<td class="day">8</td>
+
-
<td class="day">9</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">10</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_11June2012">11</a></td>
+
-
<td class="day">12</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_13June2012">13</a></td>
+
-
<td class="day">14</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_15June2012">15</a></td>
+
-
<td class="day">16</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">17</td>
+
-
<td class="day">18</td>
+
-
<td class="day">19</td>
+
-
<td class="day">20</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_21June2012">21</a></td>
+
-
<td class="day">22</td>
+
-
<td class="day">23</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">24</td>
+
-
<td class="day">25</td>
+
-
<td class="day">26</td>
+
-
<td class="day">27</td>
+
-
<td class="day">28</td>
+
-
<td class="day">29</td>
+
-
<td class="day">30</td>
+
-
</tr>
+
-
</table>
+
-
</td>
+
-
<tr>
+
-
</tr>
+
-
<td colspan=4>
+
-
<table style="margin: 0 auto; background-color: transparent;">
+
-
<tr>
+
-
<td>
+
-
<table class="calendarheader">
+
-
<tr class="monthheader">
+
-
<td colspan=7 >
+
-
<div class="mte">
+
-
<div class="mtd">
+
-
<mh>July</mh><br>
+
-
</div>
+
-
</div>
+
-
</td>
+
-
</tr>
+
-
<tr class="dayheader">
+
-
<td class="daylabelend">Su</td>
+
-
<td class="daylabel">M</td>
+
-
<td class="daylabel">Tu</td>
+
-
<td class="daylabel">W</td>
+
-
<td class="daylabel">Th</td>
+
-
<td class="daylabel">F</td>
+
-
<td class="daylabelend">Sa</td>
+
-
</tr>
+
-
</table>
+
-
<table class="calendar">
+
-
<tr>
+
-
<td class="day">1</td>
+
-
<td class="day">2</td>
+
-
<td class="day">3</td>
+
-
<td class="day">4</td>
+
-
<td class="day">5</td>
+
-
<td class="day">6</td>
+
-
<td class="day">7</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">8</td>
+
-
<td class="day">9</td>
+
-
<td class="day">10</td>
+
-
<td class="day">11</td>
+
-
<td class="day">12</td>
+
-
<td class="day">13</td>
+
-
<td class="day">14</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">15</td>
+
-
<td class="day">16</td>
+
-
<td class="day">17</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_18July2012">18</a></td>
+
-
<td class="day">19</td>
+
-
<td class="day">20</td>
+
-
<td class="day">21</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">22</td>
+
-
<td class="day">23</td>
+
-
<td class="day">24</td>
+
-
<td class="day">25</td>
+
-
<td class="day">26</td>
+
-
<td class="day">27</td>
+
-
<td class="day">28</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">29</td>
+
-
<td class="day">30</td>
+
-
<td class="day">31</td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
</tr>
+
-
</table>
+
-
</td>
+
-
<td>
+
-
<table class="calendarheader">
+
-
<tr class="monthheader">
+
-
<td colspan=7 >
+
-
<div class="mte">
+
-
<div class="mtd">
+
-
<mh>August</mh><br>
+
-
</div>
+
-
</div>
+
-
</td>
+
-
</tr>
+
-
<tr class="dayheader">
+
-
<td class="daylabelend">Su</td>
+
-
<td class="daylabel">M</td>
+
-
<td class="daylabel">Tu</td>
+
-
<td class="daylabel">W</td>
+
-
<td class="daylabel">Th</td>
+
-
<td class="daylabel">F</td>
+
-
<td class="daylabelend">Sa</td>
+
-
</tr>
+
-
</table>
+
-
<table class="calendar">
+
-
<tr>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_1August2012">1</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_2August2012">2</a></td>
+
-
<td class="day">3</td>
+
-
<td class="day">4</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">5</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_6August2012">6</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_7August2012">7</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_8August2012">8</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_9August2012">9</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_10August2012">10</a></td>
+
-
<td class="day">11</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">12</td>
+
-
<td class="day">13</td>
+
-
<td class="day">14</td>
+
-
<td class="day">15</td>
+
-
<td class="day">16</td>
+
-
<td class="day">17</td>
+
-
<td class="day">18</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">19</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_20August2012">20</a></td>
+
-
<td class="day">21</td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_22August2012">22</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_23August2012">23</a></td>
+
-
<td class="daylink"><a class="daylinka" href="http://2012.igem.org/Team:Groningen/Notebook/PublicRelations_24August2012">24</a></td>
+
-
<td class="day">25</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">26</td>
+
-
<td class="day">27</td>
+
-
<td class="day">28</td>
+
-
<td class="day">29</td>
+
-
<td class="day">30</td>
+
-
<td class="day">31</td>
+
-
<td class="day"> </td>
+
-
</tr>
+
-
</table>
+
-
</td>
+
-
<td>
+
-
<table class="calendarheader">
+
-
<tr class="monthheader">
+
-
<td colspan=7 >
+
-
<div class="mte">
+
-
<div class="mtd">
+
-
<mh>September</mh><br>
+
-
</div>
+
-
</div>
+
-
</td>
+
-
</tr>
+
-
<tr class="dayheader">
+
-
<td class="daylabelend">Su</td>
+
-
<td class="daylabel">M</td>
+
-
<td class="daylabel">Tu</td>
+
-
<td class="daylabel">W</td>
+
-
<td class="daylabel">Th</td>
+
-
<td class="daylabel">F</td>
+
-
<td class="daylabelend">Sa</td>
+
-
</tr>
+
-
</table>
+
-
<table class="calendar">
+
-
<tr>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
+
-
<td class="day"> </td>
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<td class="day">1</td>
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-
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+
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<td class="day">2</td>
+
-
<td class="day">3</td>
+
-
<td class="day">4</td>
+
-
<td class="day">5</td>
+
-
<td class="day">6</td>
+
-
<td class="day">7</td>
+
-
<td class="day">8</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">9</td>
+
-
<td class="day">10</td>
+
-
<td class="day">11</td>
+
-
<td class="day">12</td>
+
-
<td class="day">13</td>
+
-
<td class="day">14</td>
+
-
<td class="day">15</td>
+
-
</tr>
+
-
<tr>
+
-
<td class="day">16</td>
+
-
<td class="day">17</td>
+
-
<td class="day">18</td>
+
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<td class="day">19</td>
+
-
<td class="day">20</td>
+
-
<td class="day">21</td>
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<td class="day">22</td>
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<td class="day">23</td>
+
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<td class="day">24</td>
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<td class="day">25</td>
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<td class="day">26</td>
+
-
<td class="day">27</td>
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<td class="day">28</td>
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<td class="day">29</td>
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{{Template:SponsorsGroningen2012}}

Revision as of 00:02, 27 September 2012





Abstract

Every year, one third of global food production -1.3 billion tons of food- is thrown away, partially due to the “best before” dating system. iGEM Groningen 2012 seeks to provide an alternative method of assessing edibility: the Food Warden. It uses an engineered strain of Bacillus subtilis to detect and report volatiles in spoiling meat. The introduced genetic construct uses a promoter to trigger a pigment coding gene. This promoter, identified by microarray analysis, is significantly upregulated in the presence of volatiles from spoiling meat. The activity of the promoter regulates the expression of the pigment reporter and will be visible to the naked eye. For safe usage of the system, spores of our engineered strain are placed into one half of a semi-permeable capsule, the second containing a calibrated amount of nutrients. Breaking the barrier between the two compartments allows germination and growth, thereby activating the spoiling-meat sensor.



Our main accomplishments


In the lab

Most importantly: we developed a construct which makes Bacillus subtilis sense spoiled meat and produce an output in the form of a yellow pigment visible by naked eye.

Design of the “Sticker”: semi-permeable capsule: bacteria are kept inside, volatiles can go through. Proof that Bacillus subtilis grows inside the sticker.

Development of BioBrick psac-cm for Bacillus subtilis.
Easy cloning in Bacillus subtilis in Biobrick fashion for the first time! Advantages: easy to check, BioBrick compatible, E. coli compatible, stable insertion into Bacillus subtilis chromosome.

Identification of spoiled meat sensors by transcriptome analysis. Usage of an innovative experimental setup which can be used to test more sensors in the future.

We made AmilCP and AmilGFP be expressed in Bacillus subtilis. These chromoproteins can be of significant value to the other Bacillus subtilis users in the BioBrick community.


Other

Smart spores activation system: inspired by "glow in the dark stick", we designed a sticker with two compartments. One compartment holds the spores, the other the nutrients; when the nutrients compartment is braked, the spores start to germinate, activating the sticker.

We explored the definition of spoiled meat and different ways to test this.

We identified various compounds present in spoiled meat by Gas Chromatography - Mass Spectrometry.

We made a model which gives insight on how to tweak the growth of Bacillus subtilis based upon literature, experimental data and flux balance analysis.


Our sponsors: