Team:Grenoble/Project/Main Results
From 2012.igem.org
Main results
In a first place, biologists and modellers worked on a simple amplification loop.
The modellers proved that a simple amplification loop could not work. There is no state where GFP is not expressed.
(Figure 1)
Modelling results confirmed that built system will work.
(Figure 3)
As you can see the paraBAD works as expected. We began the construction of the amplification loop (paraBAD_gfp_cyaA).
A possible track would be to add another regulation system on the adenylate cyclase production with the riboswitch RsmA/rsmY.
References
[1]Accumulation of Toxic Concentrations of Methylglyoxal by Wild-Type Escherichia coli K-12, Rollie S. Ackerman, Nicholas R. Cozzarelli and Wolfgang Epstein[2]Metabolic flux analysis of Escherichia coli in glucose-limited continuous culture. II. Dynamic response to famine and feast, activation of the methylglyoxal pathway and oscillatory behaviour Jan Weber†, Anke Kayser‡ and Ursula Rinas