Team:Grenoble/Project/Main Results
From 2012.igem.org
Main results
In a first place, biologists and modellers worked on a simple amplification loop.
The modellers proved that a simple amplification loop could not work. There is no state where GFP is not expressed. (Figure 1).
Modelling results confirmed that built system will work. (Figure2)
This can be attributed to the fact that overproduction of cAMP is lethal to Escherichia coli possibly due to an accumulation of methylglyoxal [http://jb.asm.org/content/119/2/357.abstract] [http://mic.sgmjournals.org/content/151/3/707.abstract].
In the future weeks (according to ours results) we will try to make the culture of our transformed bacteria in a medium complement with glucose and acetate. A possible track would be to add another regulation system on the adenylate cyclase production with the riboswitch RsmA/rsmY.