Team:Grenoble/Project/Main Results
From 2012.igem.org
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In a first place, biologists and modellers worked on a simple amplification loop.<br/> | In a first place, biologists and modellers worked on a simple amplification loop.<br/> | ||
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- | <img src="https://static.igem.org/mediawiki/2012/d/d1/Cbe_ampli.png" alt="" /> | + | <center><img src="https://static.igem.org/mediawiki/2012/d/d1/Cbe_ampli.png" alt="" /></center> |
The modellers proved that a simple amplification loop could not work. There is no state where GFP is not expressed. | The modellers proved that a simple amplification loop could not work. There is no state where GFP is not expressed. | ||
</section> | </section> | ||
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The team thought that an AND gate could be our solution. It could add a biological noise filter and resolve the problem of the continuous expression of GFP.<br/> | The team thought that an AND gate could be our solution. It could add a biological noise filter and resolve the problem of the continuous expression of GFP.<br/> | ||
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- | <img src="https://static.igem.org/mediawiki/2012/f/f3/Graphe3_ampli_grenoble.png" alt="" /> | + | <center><img src="https://static.igem.org/mediawiki/2012/f/f3/Graphe3_ampli_grenoble.png" alt="" /></center> |
Modelling results confirmed that built system will work. | Modelling results confirmed that built system will work. | ||
</section> | </section> |
Revision as of 23:38, 26 September 2012
Main results
In a first place, biologists and modellers worked on a simple amplification loop.
(Figure 3)
As you can see the paraBAD works as expected. We began the construction of the amplification loop (paraBAD_gfp_cyaA).
A possible track would be to add another regulation system on the adenylate cyclase production with the riboswitch RsmA/rsmY.
References
[1]Accumulation of Toxic Concentrations of Methylglyoxal by Wild-Type Escherichia coli K-12, Rollie S. Ackerman, Nicholas R. Cozzarelli and Wolfgang Epstein[2]Metabolic flux analysis of Escherichia coli in glucose-limited continuous culture. II. Dynamic response to famine and feast, activation of the methylglyoxal pathway and oscillatory behaviour Jan Weber†, Anke Kayser‡ and Ursula Rinas