Team:Grenoble/Project/Abstract

From 2012.igem.org

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<h1>Project Description</h1>
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<h1>sEnsiColi: A tunable and reliable ultra-sensitive detector</h1>
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The Grenoble iGEM team intends to build an ultra sensitive detector. The goal is to produce a detector that can hugely amplify a given signal. It consists of three modules:<br/>
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Multi-resistant bacteria are a worldwide issue which in a very near future will have
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<br/>
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huge impacts on our societies and ways diagnosis and prevention will be performed.
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In this optic, the Grenoble iGEM team has built an ultra-sensitive pathogen detector.  
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<br/><br/>It consists of three interconnected modules:
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    <li>the first gets the signal</li>
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<li>Detection</li>
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    <li>the second amplifies it</li>
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<li>Amplification/Communication</li>
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    <li>the third sends the output</li>
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<li>Output</li>
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The detection module is a membrane receptor that once activated by a chemical compound actuates an amplification loop. The loop enhances the cell response efficiency.<br/>
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The detection module consists of a recombinant membrane receptor that, once activated, actuates an amplification loop. The amplification system contains a genetic feed forward loop, which filters out false  
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The amplification system contains a genetic feed forward loop. It acts as a biologic loud filter aiming at reducing false positive outputs.  
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positive outputs. Once amplified and filtered, the signal is transmitted to neighboring  
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Once amplified and filtered the signal is transmitted to the neighboring bacteria.  
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bacteria via a diffusible molecule. In turn, the amplification loop is triggered which
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In this way a noticeable fluorescent emission is generated. <br/>
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leads to the production of a measurable fluorescence output. <br/>
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<br/>The design of our
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<h4>Scheme of the concept</h4>
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network is easily adaptable to different input signals by using other receptor domains.
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<img src="http://2012.igem.org/wiki/images/9/9d/Schema1.png" alt="Scheme of the concept" style="horizontal-align: center;"/>
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<center><img src="http://2012.igem.org/wiki/images/9/9d/Schema1.png" alt="Scheme of the concept" style="horizontal-align: center;"/></center>
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<h4>Theoretical response of the device depending on the input signal</h4>
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<img src="http://2012.igem.org/wiki/images/c/cf/Schema2.png" alt="Theoretical response of the device depending on the input signal" />
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<center> <h4>Theoretical response of the device depending on the input signal</h4> <br/>
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<img src="http://2012.igem.org/wiki/images/c/cf/Schema2.png" alt="Theoretical response of the device depending on the input signal" style="horizontal-align: center;"/></center> -->
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Latest revision as of 00:59, 25 September 2012

iGEM Grenoble 2012

Project

sEnsiColi: A tunable and reliable ultra-sensitive detector

Multi-resistant bacteria are a worldwide issue which in a very near future will have huge impacts on our societies and ways diagnosis and prevention will be performed. In this optic, the Grenoble iGEM team has built an ultra-sensitive pathogen detector.

It consists of three interconnected modules:
  1. Detection
  2. Amplification/Communication
  3. Output

The detection module consists of a recombinant membrane receptor that, once activated, actuates an amplification loop. The amplification system contains a genetic feed forward loop, which filters out false positive outputs. Once amplified and filtered, the signal is transmitted to neighboring bacteria via a diffusible molecule. In turn, the amplification loop is triggered which leads to the production of a measurable fluorescence output.

The design of our network is easily adaptable to different input signals by using other receptor domains.