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Team:Grenoble/Biology/Protocols/Medium
From 2012.igem.org
(Difference between revisions)
| Line 9: | Line 9: | ||
<br/> | <br/> | ||
<section> | <section> | ||
| - | <h2>LB medium</h2> | + | <h2>LB (Lysogeny Broth) medium</h2> |
| + | <br/> | ||
| + | <table> | ||
| + | <tr> | ||
| + | <td>Tryptone</td> | ||
| + | <td>10 g/L</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Yeast extract</td> | ||
| + | <td>5 g/L</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>NaCl</td> | ||
| + | <td>5 g/L</td> | ||
| + | </tr> | ||
| + | </table> | ||
</section> | </section> | ||
Revision as of 20:37, 22 August 2012
Medium composition
LB (Lysogeny Broth) medium
| Tryptone | 10 g/L |
| Yeast extract | 5 g/L |
| NaCl | 5 g/L |
M9 medium
Add ingredients in the following order:| H2O | 396.2 mL |
| CaCl2 (1M) | 50 µL |
| MgSO4 (1M) | 1 mL |
| Salts (5X) | 100 mL |
| Vitamine B1 (1%) | 250 µL |
The composition for a 2X solution of the salts is the following:
| Amount for 1L in g | FW | |
|---|---|---|
| Na2HPO4 x 2H2O | 13.8 | 141.96 |
| KH2PO4 | 6.1 | 136.09 |
| NaCl | 1.0 | 58.44 |
| NH4Cl | 4.1 | 53.49 |
