Team:Grenoble/Biology/Protocols/Medium
From 2012.igem.org
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Revision as of 20:38, 22 August 2012
Medium composition
LB (Lysogeny Broth) medium
Tryptone | 10 g/L |
Yeast extract | 5 g/L |
NaCl | 5 g/L |
M9 medium
Add ingredients in the following order:H2O | 396.2 mL |
CaCl2 (1M) | 50 µL |
MgSO4 (1M) | 1 mL |
Salts (5X) | 100 mL |
Vitamine B1 (1%) | 250 µL |
The composition for a 2X solution of the salts is the following:
Amount for 1L in g | FW | |
---|---|---|
Na2HPO4 x 2H2O | 13.8 | 141.96 |
KH2PO4 | 6.1 | 136.09 |
NaCl | 1.0 | 58.44 |
NH4Cl | 4.1 | 53.49 |