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Team:Grenoble/Biology/Protocols/Medium
From 2012.igem.org
(Difference between revisions)
| Line 20: | Line 20: | ||
<table> | <table> | ||
<tr> | <tr> | ||
| - | <td>H<span class="indice">2</span>O</td> | + | <td> H<span class="indice">2</span>O</td> |
| - | <td>396.2 mL</td> | + | <td> 396.2 mL</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>CaCl<span class="indice">2</span> (1M)</td> | + | <td> CaCl<span class="indice">2</span> (1M)</td> |
| - | <td>50 µL</td> | + | <td> 50 µL</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>MgSO<span class="indice">4</span> (1M)</td> | + | <td> MgSO<span class="indice">4</span> (1M)</td> |
| - | <td>1 mL</td> | + | <td> 1 mL</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Salts (5X)</td> | + | <td> Salts (5X)</td> |
| - | <td>100 mL</td> | + | <td> 100 mL</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Vitamine B1 (1%) </td> | + | <td> Vitamine B1 (1%) </td> |
| - | <td>250 µL</td> | + | <td> 250 µL</td> |
</table> | </table> | ||
<br/> | <br/> | ||
Revision as of 20:10, 22 August 2012
Medium composition
LB medium
M9 medium
Add ingredients in the following order:| H2O | 396.2 mL |
| CaCl2 (1M) | 50 µL |
| MgSO4 (1M) | 1 mL |
| Salts (5X) | 100 mL |
| Vitamine B1 (1%) | 250 µL |
The composition for a 2X solution of the salts is the following:
| Amount for 1L in g | FW | |
|---|---|---|
| Na2HPO4 x 2H2O | 13.8 | 141.96 |
| KH2PO4 | 6.1 | 136.09 |
| NaCl | 1.0 | 58.44 |
| NH4Cl | 4.1 | 53.49 |
