Team:Grenoble/Biology/Protocols/Medium
From 2012.igem.org
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<br/> | <br/> | ||
<section> | <section> | ||
- | <h2>LB medium</h2> | + | <h2>LB (Lysogeny Broth) medium</h2> |
+ | <br/> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <td> Tryptone</td> | ||
+ | <td> 10 g/L </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> Yeast extract </td> | ||
+ | <td> 5 g/L</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> NaCl</td> | ||
+ | <td> 5 g/L</td> | ||
+ | </tr> | ||
+ | </table> | ||
</section> | </section> | ||
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<tr> | <tr> | ||
<td> H<span class="indice">2</span>O</td> | <td> H<span class="indice">2</span>O</td> | ||
- | <td> 396.2 mL</td> | + | <td> 396.2 mL </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<tr> | <tr> | ||
<td></td> | <td></td> | ||
- | <th>Amount for 1L in g</th> | + | <th> Amount for 1L (in g) </th> |
- | <th>FW</th> | + | <th> FW</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> Na<span class="indice">2</span>HPO<span class="indice">4</span> x 2H<span class="indice">2</span>O </td> | <td> Na<span class="indice">2</span>HPO<span class="indice">4</span> x 2H<span class="indice">2</span>O </td> | ||
<td> 13.8</td> | <td> 13.8</td> | ||
- | <td> 141.96</td> | + | <td> 141.96 </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> KH<span class="indice">2</span>PO<span class="indice">4</span></td> | <td> KH<span class="indice">2</span>PO<span class="indice">4</span></td> | ||
<td> 6.1</td> | <td> 6.1</td> | ||
- | <td> 136.09</td> | + | <td> 136.09 </td> |
</tr> | </tr> | ||
<tr> | <tr> |
Latest revision as of 08:14, 25 September 2012
Medium composition
LB (Lysogeny Broth) medium
Tryptone | 10 g/L |
Yeast extract | 5 g/L |
NaCl | 5 g/L |
M9 medium
Add ingredients in the following order:H2O | 396.2 mL |
CaCl2 (1M) | 50 µL |
MgSO4 (1M) | 1 mL |
Salts (5X) | 100 mL |
Vitamine B1 (1%) | 250 µL |
The composition for a 2X solution of the salts is the following:
Amount for 1L (in g) | FW | |
---|---|---|
Na2HPO4 x 2H2O | 13.8 | 141.96 |
KH2PO4 | 6.1 | 136.09 |
NaCl | 1.0 | 58.44 |
NH4Cl | 4.1 | 53.49 |