Team:Grenoble/Biology/Protocols/Medium

From 2012.igem.org

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<br/>
<br/>
<section>
<section>
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     <h2>LB medium</h2>
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     <h2>LB (Lysogeny Broth) medium</h2>
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    <br/>
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    <table>
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        <tr>
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            <td>&nbsp;Tryptone</td>
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            <td>&nbsp;10 g/L&nbsp;</td>
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        </tr>
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        <tr>
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            <td>&nbsp;Yeast extract&nbsp;</td>
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            <td>&nbsp;5 g/L</td>
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        </tr>
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        <tr>
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            <td>&nbsp;NaCl</td>
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            <td>&nbsp;5 g/L</td>
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        </tr>
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    </table>
      
      
</section>
</section>
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     <br/>
     <br/>
     <br/>
     <br/>
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     <table>
     <table>
     <tr>
     <tr>
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         <td>H2O</td>
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         <td>&nbsp;H<span class="indice">2</span>O</td>
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         <td>396.2 mL</td>
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         <td>&nbsp;396.2 mL&nbsp;</td>
     </tr>
     </tr>
     <tr>
     <tr>
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         <td>CaCl2 (1M)</td>
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         <td>&nbsp;CaCl<span class="indice">2</span> (1M)</td>
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         <td>50 µL</td>
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         <td>&nbsp;50 µL</td>
     </tr>
     </tr>
     <tr>
     <tr>
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         <td>MgSO4 (1M)</td>
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         <td>&nbsp;MgSO<span class="indice">4</span> (1M)</td>
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         <td>1 mL</td>
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         <td>&nbsp;1 mL</td>
     </tr>
     </tr>
     <tr>
     <tr>
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         <td>Salts (5X)</td>
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         <td>&nbsp;Salts (5X)</td>
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         <td>100 mL</td>
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         <td>&nbsp;100 mL</td>
     </tr>
     </tr>
     <tr>
     <tr>
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         <td>Vitamine B1 (1%)&nbsp;&nbsp;</td>
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         <td>&nbsp;Vitamine B1 (1%)&nbsp;&nbsp;</td>
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         <td>250 µL</td>
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         <td>&nbsp;250 µL</td>
     </table>
     </table>
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    <br/>
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    <br/>
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    The composition for a <b>2X</b> solution of the salts is the following:
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    <br/>
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    <br/>
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    <table>
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    <tr>
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        <td></td>
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        <th>&nbsp;Amount for 1L (in g)&nbsp;</th>
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        <th>&nbsp;FW</th>
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    </tr>
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    <tr>
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        <td>&nbsp;Na<span class="indice">2</span>HPO<span class="indice">4</span> x 2H<span class="indice">2</span>O&nbsp;&nbsp;</td>
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        <td>&nbsp;13.8</td>
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        <td>&nbsp;141.96&nbsp;</td>
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    </tr>
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    <tr>
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        <td>&nbsp;KH<span class="indice">2</span>PO<span class="indice">4</span></td>
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        <td>&nbsp;6.1</td>
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        <td>&nbsp;136.09&nbsp;</td>
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    </tr>
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    <tr>
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        <td>&nbsp;NaCl</td>
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        <td>&nbsp;1.0</td>
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        <td>&nbsp;58.44</td>
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    </tr>
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    <tr>
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        <td>&nbsp;NH<span class="indice">4</span>Cl</td>
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        <td>&nbsp;4.1</td>
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        <td>&nbsp;53.49</td>
 +
    </table>
 +
   
      
      
</section>
</section>

Latest revision as of 08:14, 25 September 2012

iGEM Grenoble 2012

Project

Medium composition


LB (Lysogeny Broth) medium


 Tryptone  10 g/L 
 Yeast extract   5 g/L
 NaCl  5 g/L

M9 medium

Add ingredients in the following order:

 H2O  396.2 mL 
 CaCl2 (1M)  50 µL
 MgSO4 (1M)  1 mL
 Salts (5X)  100 mL
 Vitamine B1 (1%)    250 µL


The composition for a 2X solution of the salts is the following:

 Amount for 1L (in g)   FW
 Na2HPO4 x 2H2O    13.8  141.96 
 KH2PO4  6.1  136.09 
 NaCl  1.0  58.44
 NH4Cl  4.1  53.49