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Team:Grenoble/Biology/Protocols/LB
From 2012.igem.org
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<h2>Prepare the LB Agar</h2> | <h2>Prepare the LB Agar</h2> | ||
In a 500mL bottle add 6.4g of bacteriological agar to 400mL of LB medium. | In a 500mL bottle add 6.4g of bacteriological agar to 400mL of LB medium. | ||
| - | <br/>Then sterelize using the autoclave. | + | <br/>Then sterelize using the autoclave.<br/> |
| + | You can store it at room temperature, but before use it, you need to heat it up. | ||
</section> | </section> | ||
<section> | <section> | ||
Revision as of 07:20, 23 August 2012
LB Agar plates preparation
Goal
Prepare solid medium to culture bacteria.Prepare the LB Agar
In a 500mL bottle add 6.4g of bacteriological agar to 400mL of LB medium.Then sterelize using the autoclave.
You can store it at room temperature, but before use it, you need to heat it up.
