Team:Grenoble/Biology/Protocols/Glycerol

From 2012.igem.org

(Difference between revisions)
Line 14: Line 14:
     You need to:
     You need to:
     <ol>
     <ol>
-
         <li>grow a liquid culture overnight</li>
+
         <li>Grow a liquid culture overnight</li>
-
         <li>prepare sterile 80% glycerol</li>
+
         <li>Prepare sterile 80% glycerol</li>
-
         <li>mix 200 µL of glycerol and 800 µL of liquid culture</li>
+
         <li>Mix 200 µL of glycerol and 800 µL of liquid culture</li>
     </ol>
     </ol>
     <br/>
     <br/>

Revision as of 14:20, 27 August 2012

iGEM Grenoble 2012

Project

Glycerol stock preparation

Goal

Prepare glycerol stock is a way to store bacteria during a long period.

Protocol

You need to:
  1. Grow a liquid culture overnight
  2. Prepare sterile 80% glycerol
  3. Mix 200 µL of glycerol and 800 µL of liquid culture

Then store the glycerol stock at -80°C.