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Team:Grenoble/Biology/Protocols/Glycerol
From 2012.igem.org
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<li>grow a liquid culture overnight</li> | <li>grow a liquid culture overnight</li> | ||
<li>prepare sterile 80% glycerol</li> | <li>prepare sterile 80% glycerol</li> | ||
| - | <li>mix 200 | + | <li>mix 200 µL of glycerol and 800 µL of liquid culture</li> |
</ol> | </ol> | ||
<br/> | <br/> | ||
Revision as of 09:53, 24 August 2012
Glycerol stock preparation
Goal
Prepare glycerol stock is a way to store bacteria during a long period.Protocol
You need to:- grow a liquid culture overnight
- prepare sterile 80% glycerol
- mix 200 µL of glycerol and 800 µL of liquid culture
Then store the glycerol stock at -80°C.
