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Team:Grenoble/Biology/Protocols
From 2012.igem.org
(Difference between revisions)
| Line 12: | Line 12: | ||
<h2>Cell culture</h2> | <h2>Cell culture</h2> | ||
<ul> | <ul> | ||
| - | <li>Medium compositions</li> | + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols">Medium compositions</a></li> |
| - | <li>LB Agar plates preparation</li> | + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols">LB Agar plates preparation</a></li> |
| - | <li>Glycerol stock production</li> | + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols">Glycerol stock production</a></li> |
</ul> | </ul> | ||
</section> | </section> | ||
| Line 20: | Line 20: | ||
<h2>BioBricks utilisation</h2> | <h2>BioBricks utilisation</h2> | ||
<ul> | <ul> | ||
| - | <li>BioBricks extraction</li> | + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols">BioBricks extraction</a></li> |
| - | <li>Chemically competent cells production</li> | + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols">Chemically competent cells production</a></li> |
| - | <li>Transformation of <i>E. coli</i> competent cells (protocol 1)</li> | + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols">Transformation of <i>E. coli</i> competent cells (protocol 1)</a></li> |
| - | <li>Transformation of <i>E. coli</i> competent cells (protocol 2)</li> | + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols">Transformation of <i>E. coli</i> competent cells (protocol 2)</a></li> |
</ul> | </ul> | ||
</section> | </section> | ||
| Line 29: | Line 29: | ||
<h2>Molecular biological techniques</h2> | <h2>Molecular biological techniques</h2> | ||
<ul> | <ul> | ||
| - | <li>PCR using a GoTaq polymerase</li> | + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols">PCR using a GoTaq polymerase</a></li> |
| - | <li>PCR using a HF Phusion polymerase</li> | + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols">PCR using a HF Phusion polymerase</a></li> |
| - | <li>Gibson Assembly</li> | + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols">Gibson Assembly</a></li> |
| - | <li>Restriction enzyme digest</li> | + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols">Restriction enzyme digest</a></li> |
| - | <li>Ligation</li> | + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols">Ligation</a></li> |
| - | <li>Miniprep</li> | + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols">Miniprep</a></li> |
</ul> | </ul> | ||
</section> | </section> | ||
| Line 40: | Line 40: | ||
<h2>Gel migration</h2> | <h2>Gel migration</h2> | ||
<ul> | <ul> | ||
| - | <li>Gel electrophoresis</li> | + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols">Gel electrophoresis</a></li> |
| - | <li>DNA extraction</li> | + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols">DNA extraction</a></li> |
</ul> | </ul> | ||
</section> | </section> | ||
| Line 47: | Line 47: | ||
<h2>Test techniques</h2> | <h2>Test techniques</h2> | ||
<ul> | <ul> | ||
| - | <li>"AND" gate test (control of pBAD expression by cAMP and arabinose)</li> | + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols">"AND" gate test (control of pBAD expression by cAMP and arabinose)</a></li> |
</ul> | </ul> | ||
</section> | </section> | ||
Revision as of 13:46, 22 August 2012
