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Team:Grenoble/Biology/Protocols
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<section> | <section> | ||
<h1>Protocols</h1> | <h1>Protocols</h1> | ||
| - | In this section you will find the description of our experiments | + | In this section you will find the description of our experiments in order to be able to reproduce what we did (if you have the will). <br/> |
| + | <br/> | ||
| + | For numerous protocols, you will find a part called <span class="petit"><i><b>SAFETY AND USEFUL RECOMMANDATION</b></i></span> which is a part of our work on the <a href="https://2012.igem.org/Team:Grenoble/Safety/Assesment">safety</a>. | ||
</section> | </section> | ||
<br/> | <br/> | ||
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<ul> | <ul> | ||
<li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/BB">BioBricks extraction</a></li> | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/BB">BioBricks extraction</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Transformation_1">Transformation of <i>E. coli</i> cells (TSS method)</a></li> | ||
<li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Competence">Chemically competent <i>E. coli</i> cells production</a></li> | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Competence">Chemically competent <i>E. coli</i> cells production</a></li> | ||
| - | |||
<li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Transformation_2">Transformation of <i>E. coli</i> competent cells (heat shock)</a></li> | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Transformation_2">Transformation of <i>E. coli</i> competent cells (heat shock)</a></li> | ||
</ul> | </ul> | ||
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<h2>Molecular biological techniques</h2> | <h2>Molecular biological techniques</h2> | ||
<ul> | <ul> | ||
| - | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/PCR_1">PCR using a GoTaq polymerase</a></li> | + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/PCR_1">PCR using a GoTaq<span class="exposant">®</span> polymerase</a></li> |
<li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/PCR_2">PCR using a HF Phusion polymerase</a></li> | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/PCR_2">PCR using a HF Phusion polymerase</a></li> | ||
<li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/GA">Gibson Assembly</a></li> | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/GA">Gibson Assembly</a></li> | ||
| - | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Restriction">Restriction enzyme | + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Restriction">Restriction enzyme digestion of DNA</a></li> |
<li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Ligation">Ligation</a></li> | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Ligation">Ligation</a></li> | ||
| - | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Miniprep">Miniprep</a></li> | + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Miniprep">Plasmid Miniprep</a></li> |
| + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Double">Double mutant construction</a></li | ||
</ul> | </ul> | ||
</section> | </section> | ||
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<ul> | <ul> | ||
<li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Gel">Gel electrophoresis</a></li> | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Gel">Gel electrophoresis</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/EtBr">Ethidium Bromide (EtBr) revelation</a></li> | ||
<li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/DNA_extract">DNA extraction from Agarose gel</a></li> | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/DNA_extract">DNA extraction from Agarose gel</a></li> | ||
</ul> | </ul> | ||
Latest revision as of 01:18, 27 September 2012
Protocols
In this section you will find the description of our experiments in order to be able to reproduce what we did (if you have the will).For numerous protocols, you will find a part called SAFETY AND USEFUL RECOMMANDATION which is a part of our work on the safety.
